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Team:Bielefeld-Germany/Labjournal/week15
From 2012.igem.org
Contents |
Week 15 (08/06 - 08/12/12)
Monday August 6th
- Team Site Directed Mutagenesis: Plasmidisolation of E.coli_2307 V+VI
- Team Cellulose Binding Domain: Plated one colonie of Bba_I13522
- Dephosphorilation of pSB1C3 Backbone
- Ligation of CBDcex+pSB1C3 and CBDclos+pSB1C3
- Transformed in KRX and plated on CM-selection-agar
- Team Bacterial Laccases
- No positive colonies after transformation of our assemblies from August 1st, but we realized that the primers we used for making the promoter parts can’t ligate with our insert an backbone because the primers are dephosphorylated and the plasmid backbone is dephosphorylated, too.
- Picking positive colonies from transformation of E.coli P/S and E. coli HIS for plasmid isolation.
Tuesday August 7th
Wednesday August 8th
Thursday August 9th
Friday August 10th
Saturday August 11th
Sunday August 12th
Sunday
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