Team:Bielefeld-Germany/Labjournal/week15
From 2012.igem.org
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**Transformed in KRX and plated on CM-selection-agar | **Transformed in KRX and plated on CM-selection-agar | ||
* '''Team Bacterial Laccases''' | * '''Team Bacterial Laccases''' | ||
- | ** No positive colonies after transformation of our assemblies from August 1st, but we realized that the primers we used for making the promoter parts can’t ligate with our insert an backbone because the primers are dephosphorylated and the plasmid backbone is dephosphorylated, too. | + | ** No positive colonies after transformation of our assemblies from August 1st, but we realized that the primers we used for making the promoter parts can’t ligate with our insert an backbone because the primers are dephosphorylated and the plasmid backbone is dephosphorylated, too. Much effort in a mission which can't work but at least we know why it doesn't work. |
- | + | :* Picking positive colonies from transformation of ''E.coli'' P/S and ''E. coli'' HIS for plasmid isolation. | |
===Tuesday August 7th=== | ===Tuesday August 7th=== |
Revision as of 22:00, 17 September 2012
Contents |
Week 15 (08/06 - 08/12/12)
Monday August 6th
- Team Site Directed Mutagenesis: Plasmidisolation of E.coli_2307 V+VI
- Team Cellulose Binding Domain: Plated one colonie of Bba_I13522
- Dephosphorilation of pSB1C3 Backbone
- Ligation of CBDcex+pSB1C3 and CBDclos+pSB1C3
- Transformed in KRX and plated on CM-selection-agar
- Team Bacterial Laccases
- No positive colonies after transformation of our assemblies from August 1st, but we realized that the primers we used for making the promoter parts can’t ligate with our insert an backbone because the primers are dephosphorylated and the plasmid backbone is dephosphorylated, too. Much effort in a mission which can't work but at least we know why it doesn't work.
- Picking positive colonies from transformation of E.coli P/S and E. coli HIS for plasmid isolation.
Tuesday August 7th
Wednesday August 8th
Thursday August 9th
Friday August 10th
Saturday August 11th
Sunday August 12th
Sunday
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