Team:Bielefeld-Germany/Labjournal/week14

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{{Team:Bielefeld/Head}}
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=Labjournal=
 
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1 Week1]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2 Week2]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3 Week3]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4 Week4]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5 Week5]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6 Week6]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7 Week7]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8 Week8]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9 Week9]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10 Week10]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11 Week11]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12 Week12]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13 Week13]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14 Week14]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15 Week15]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16 Week16]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17 Week17]
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[https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18 Week18]
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==Week 14 (07/30 - 08/05/12)==
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===Monday July 30th===
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* '''Team Site Directed Mutagenesis:''' Plasmid-isolations of plated colonies
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<div id="nav" class="tabs">
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* '''Team Cellulose Binding Domain:''' Redesigned the primer-sequences another time giving the CBDs a few AS more in than the Protein-BLAST said. CBDcex: 4 AS N-terminal, 2 C-terminal. CBDclos: 2 N-terminal (starting with a natural ATG) and 2 AS C-terminal.
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**Prepared  BBa_392014 for Sequencing
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<ul style="list-style-type:none">
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/Starting"><strong>Prologue</strong></a></li>
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                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week1"><strong>Week 1</strong></a></li>
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                <li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week2"><strong>Week 2</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week3"><strong>Week 3</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week4"><strong>Week 4</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week5"><strong>Week 5</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6"><strong>Week 6</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week7"><strong>Week 7</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week8"><strong>Week 8</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week9"><strong>Week 9</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week10"><strong>Week 10</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week11"><strong>Week 11</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week12"><strong>Week 12</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week13"><strong>Week 13</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week14"><strong>Week 14</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week15"><strong>Week 15</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week16"><strong>Week 16</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week17"><strong>Week 17</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week18"><strong>Week 18</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week19"><strong>Week 19</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week20"><strong>Week 20</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week21"><strong>Week 21</strong></a></li>
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<li><a href="https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week22"><strong>Week 22</strong></a></li>
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===Tuesday July 31st===
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</ul>
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* '''Team Site Directed Mutagenesis:''' More plasmid-isolations, test-restriktions and colony-pickings.
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===Wednesday August 1st===
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</div>
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===Thursday August 2nd===
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</div>
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* '''Team Wiki:''' This morning we met for taking a new picture of our group and individual pictures of everyone. Check out our beautiful team members [https://2012.igem.org/Team:Bielefeld-Germany/Students here].
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</html>
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===Friday August 3rd===
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<div style="text-align:justify;">
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* '''Team Site Directed Mutagenesis:''' Transformed ''T.vers''_243_III_1161 and ''X.camp.''_2K_2 V_3633 in XL1 Blue and plated it on Ampicillin- and CM-selection-agar.
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==Week 14 (07/30 - 08/05/12)==
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* '''Team Cellulose Binding Domain:''' Transformed the plasmids p714_cex and 570_clos we got from the fermantationgroup in KRX an plated them on Kanamycin-selectionagar.
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===Saturday August 4th===
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* '''Team Site Directed Mutagenesis:''' Plated four isolated colonies from ''T.vers''_243_III_1161 and ''X.camp.''_2K_2 V_3633 per petri dish on seperated selection-agar-dishes
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* '''Team Cellulose Binding Domain:''' Since there were only a few colonies on the p570 selection-agar-dish, I picked one, resuspended it in 100 µL destilled water and plated it on an new Kanamycin-selection-agar-dish
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**plasmid-isolation of p714
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===Sunday August 5th===
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{{Team:Bielefeld/Sponsoren}}
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* '''Team Site Directed Mutagenesis:''' Plasmidisolations of ''X.camp.''2K_2_3633 (I-IV) and T.vers_243_III_1161 (I-IV)
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**Test-Restriktion of ''X.camp.''2K_2_3633 (I-IV) and ''T.vers''_243_III_1161 (I-IV) X.camp III + IV good; T.vers, no positiv, since the 1150 band should at least rise to 1550
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**Picked two additional colonies of ''e.coli''_2307 and plated them on CM
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**Prepared  ''X.camp.''2K_2_3633 III + IV for sequencing
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* '''Team Cellulose Binding Domain:''' Transformation of BBa_I13522 + pSB1A2 in KRX and plating it on AMP-Selection-Agar
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**Plasmidisolations of p570
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**PCR of CBDcex (417 bp) and CBDclos (369 bp)
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***Test-Gelelectrophoresis: Got the bands that should be there!
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***PCR Clean up (no Gelelectrophoresis)
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**Restriktion of PCR-products and pSB1C3_RFP with XbaI and PstI.
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Latest revision as of 21:24, 25 September 2012


Week 14 (07/30 - 08/05/12)

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About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

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