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From 2012.igem.org

Contents 1 Labjournal 1.1 Week 12 (07/16 - 07/22/12) 1.1.1 Monday July 16th 1.1.2 Tuesday July 17th 1.1.3 Wednesday July 18th 1.1.4 Thursday July 19th 1.1.5 Friday July 20th 1.1.6 Saturday July 21st 1.1.7 Sunday July 22nd

Labjournal

Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18

Week 12 (07/16 - 07/22/12)

Monday July 16th

• Team A. thaliana laccase: Our plants had a great time during the last weeks in the climate chamber. So today it was time for them to donate their seeds for RNA isolation, cDNA synthesis and a PCR (check protocols). We ran an additional sample with actin primers as a positive control. However both samples did not show any bands. Maybe the high salt concentration in our sample was responsible or the laccase concentration in the 1:10 deluted cDNA was too low. We will do some washing and try again.

Tuesday July 17th

• Team Cultivation/Purification: Second cultivation
• Team Site Directed Mutagenesis: Made Clone Manager files for Trametes versicolor sequences

Wednesday July 18th

• Team Site Directed Mutagenesis: Made Primer-Mixes. Got everything ready for Lab work.

Thursday July 19th

Friday July 20th

• Team Modeling: We need a contact to a clarification plant to get information about clarification plant itself and perhaps to proof our cleaner with real probes. therefor we are calling Mr. Bülter form the clarification plant Schloß Holte (near Bielefeld) and he invited us to present our projekt.

Saturday July 21st

Sunday July 22nd