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Team:Bielefeld-Germany/Labjournal/week12
From 2012.igem.org
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| + | *'''Team Cultivation/Purification''': Second cultivation | ||
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===Thursday July 19th=== | ===Thursday July 19th=== | ||
Revision as of 18:54, 12 August 2012
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15
Week 12 (07/16 - 07/22/12)
Monday July 16th
- Team A. thaliana laccase: Our plants had a great time during the last weeks in the climate chamber. So today it was time for them to donate their seeds for RNA isolation, cDNA synthesis and a PCR (check protocols). We ran an additional sample with actin primers as a positive control. However both samples did not show any bands. Maybe the high salt concentration in our sample was responsible or the laccase concentration in the 1:10 deluted cDNA was too low. We will do some washing and try again.
Tuesday July 17th
- Team Cultivation/Purification: Second cultivation
