Team:Alberta

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Welcome to the 2012 Alberta iGEM team Wiki!
Welcome to the 2012 Alberta iGEM team Wiki!
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This wiki is still under construction, and as such may contain missing pages or information. Do not grieve, we are still working on expanding it.
This wiki is still under construction, and as such may contain missing pages or information. Do not grieve, we are still working on expanding it.

Revision as of 00:24, 2 October 2012




Welcome to the 2012 Alberta iGEM team Wiki!
rinbow picture This wiki is still under construction, and as such may contain missing pages or information. Do not grieve, we are still working on expanding it.

 Towards a Microbial Color Wheel: Spatial Control of Gene Expression

Abstract

As a young team composed of high schoolers and junior undergraduates, we selected a project aimed at giving ourselves a firm understanding in the fundamentals of genetic engineering and control. The end goal of our project was to create spatial color patterns using bacteria, such as a color wheel and a rainbow, that required control over several color outputs in response to spatial gradients of chemical inducers. Colour gradients were achieved using a high-copy plasmid that contained both an inducible colour gene and its corresponding repressor. Colour banding was achieved by a novel means of adjusting gene expression through plasmid copy number control that varied from 0 to ~1000 copies/cell as a function of inducer concentration. Note that the rapid loss of plasmid that occurs in the absence of inducer also constitutes a novel and extremely effective safety switch for genetically engineered organisms which might enter the environment.

Finished work

description

Future plans

description

2012 Sponsors
                Alberta innovates             Geneious             IDT

                NEB             OSLI             U oF A

Site Map

Home Team Project Parts Attributions Notebook Human Practices Outreach Sponsors
Promoter Repressor
Reporter Data Page
The Future References
Plasmid # control
Chemical Gradient