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Team:Bielefeld-Germany/Labjournal/week17
From 2012.igem.org
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Labjournal
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Week 17 (08/20 - 08/26/12)
Monday August 20th
- Team Site Directed Mutagenesis:
Plasmidisolation of X.camp_2247 1-4 and X.camp_3633 1-4 Testrestriktion with PstI: - all negativ Colony-PCR of T10_NotI (24 Colonies) -all negativ
- Team Cellulose Binding Domain:
CBD: Colony-PCR of 24 Assembly-colonies - all colonies only have CBDclos. Assembly didn't work at all...
Tuesday August 21st
Restriktion of pSB1C3+CBDclos with EcoRI+AgeI-HF in Buffer 4 + BSA To test the Restriktion Enzymes - works
Wednesday August 22nd
Street Science: Went to Dr. Joe Max Risse today and asked him about the mircoorganisms of the Fermentationgroup. He recommended Euglena gracilis, since it is without risk, big, colorful, and very healthy under the microscop. I asked for the bacteria they have and he told me I could check if their Kocuria rosea is a wild type. In the DMSZ catalog there are three strains of Kocuria rosea and all have been assessed to be of riskgroup 1, which is the safest group of bacteria and means it is unlikely that it will infect humans. I also asked for Penicillium chrysogenum but the one they use is a GVO.
- Team Site Directed Mutagenesis:
Ordered new primers for Xantomonas Campestris SDM, since there is still no positiv coloniy and even gradient-PCR gave the wrong product
- Team Cellulose Binding Domain:
Test-restriktion of all isolated CBDcex- and the GFP_Freiburg-plasmids with NotI - no positiv result Started new and made a gradient PCR for GFP-Freiburg and CBDcex (50-70°C)
