"
Team:Bielefeld-Germany/Labjournal/week13
From 2012.igem.org
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15
Week 13 (07/23 - 07/29/12)
Monday July 23rd
- Some of our team members were at CAS conference in Munich from 07/23 - 07/25/12.
- Successful PCRs of laccase gene lac5 from Trametes versicolor with plasmid DNA from Uni Greifswald as template.
Tuesday July 24th
- Team Activity Tests: Today was the moment of truth. We received the first recombinant laccases from the cultivation team. We started with testing the supernatant from the cultivated cells to check whether they secrete laccase. We added 0,1 mM ABTS in each well that was filled with supernatant from Escherichia coli, Bacillus pumilus, Bacillus halodurans C-125, Xanthomonas campestris pv. campestris B100 and E. coli KRX (negative control - cells without plasmid) and measured as usual. There was no change in the OD so that we assume that no secretion takes place. Afterwards we tested potential laccase activity by filling each well with laccase and buffer (check protocols to see which buffer we used) and added 0,1 mM ABTS. Unfortunately no activity was seen. We will try again soon.
