Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''E. coli'' BL21-Laccase (CueO named ECOL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/coli Results]  
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''E. coli'' BL21-Laccase (CueO named ECOL).  
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/coli Results]  
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Trametes versicolor''-Laccase (named TVEL0) and measured their activity. [https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Trametes versicolor''-laccase (named TVEL0) and measured their activity.  
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''E. coli'' BL21-Laccase (CueO named ECOL) and measured their activity. [https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''E. coli'' BL21-laccase (CueO named ECOL) and measured their activity.  
 +
[https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Bacillus pumilus'' DSM 27-Laccase (CotA named BPUL) and measured their activity. [https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully immobilized ''Bacillus pumilus'' DSM 27-laccase (CotA named BPUL) and measured their activity. [https://2012.igem.org/Team:Bielefeld-Germany/Results/immo Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully created a modell which simulates the oxydation of ABTS. [https://2012.igem.org/Team:Bielefeld-Germany/Modell Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully created a model which simulates the oxidation of ABTS.
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[https://2012.igem.org/Team:Bielefeld-Germany/Modell Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We conducted a feasability study of our project with sewage plant experts [https://2012.igem.org/Team:Bielefeld-Germany/Sewage_treatment_plant Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We conducted a feasibility study of our project with sewage plant experts.
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[https://2012.igem.org/Team:Bielefeld-Germany/Sewage_treatment_plant Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We did an outstanding outreach work [https://2012.igem.org/Team:Bielefeld-Germany/Public_relations_Overview Results]
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[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We did an outstanding outreach work.
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[https://2012.igem.org/Team:Bielefeld-Germany/Public_relations_Overview Results]

Revision as of 12:36, 20 October 2012


Project Achievements

Bielefeld2012 haken grau.jpg
We successfully produced purified and identified an active E. coli BL21-Laccase (CueO named ECOL).

Results


Bielefeld2012 haken grau.jpg
We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest. Results


Bielefeld2012 haken grau.jpg
We successfully produced purified and identified an active Bacillus pumilus DSM 27-Laccase (CotA named BPUL). Results


Bielefeld2012 haken grau.jpg
We successfully produced, purified an active Thermus thermophilus HB27-Laccase (tthL named TTHL). Results


Bielefeld2012 haken grau.jpg
We successfully produced an active Bacillus halodurans C-125e-Laccase (Lbh1 named BHAL). Results


Bielefeld2012 haken grau.jpg
Successful characterization of the activity of B. pumilus Laccase regarding the oxidization of ABTS under different conditions. Results


Bielefeld2012 haken grau.jpg
Successful characterization of the activity of E. coli Laccase regarding the oxidization of ABTS under different conditions. Results


Bielefeld2012 haken grau.jpg
Successful measurements of enzyme activity of T. thermophilus Laccase. Results


Bielefeld2012 haken grau.jpg
Successful measurements of enzyme activity of B. halodurans Laccase. Results


Bielefeld2012 haken grau.jpg
We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol. Results


Bielefeld2012 haken grau.jpg
We successfully immobilized Trametes versicolor-laccase (named TVEL0) and measured their activity.

Results


Bielefeld2012 haken grau.jpg
We successfully immobilized E. coli BL21-laccase (CueO named ECOL) and measured their activity.

Results


Bielefeld2012 haken grau.jpg
We successfully immobilized Bacillus pumilus DSM 27-laccase (CotA named BPUL) and measured their activity. Results


Bielefeld2012 haken grau.jpg
We successfully improved a BioBrick by clarifying its true function. [http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]


Bielefeld2012 haken grau.jpg
We successfully created our own database to organize our samples. Results


Bielefeld2012 haken grau.jpg
We successfully created a model which simulates the oxidation of ABTS.

Results


Bielefeld2012 haken grau.jpg
We conducted a feasibility study of our project with sewage plant experts.

Results


Bielefeld2012 haken grau.jpg
We did an outstanding outreach work.

Results



55px Logo merck.jpg BioCircle.JPG Bielefeld2012 Evonik.jpg Bielefeld2012 Baxter.png Logo knauer.jpg Logo iit.jpg Bielefeld2012 BIEKUBA.jpg Logo biometra.jpg Logo bio-nrw.png Bielefeld2012 Logo ERASynbio.jpg