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Team:TMU-Tokyo/Notebook/Experiment
From 2012.igem.org
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<b>■Week 0(8.9-8.11)</b><Br> | <b>■Week 0(8.9-8.11)</b><Br> | ||
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Cultivation of the E.coli on LB culture<Br></p> | Cultivation of the E.coli on LB culture<Br></p> | ||
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Revision as of 14:25, 26 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
Plasmid DNA Purification
Genome DNA Purification
Restruction Enzyme Degestion
DNA Fragment Ligation
Transformation
Electrophoresis
LB Medium
■Assay
Device1 Assay
Device2 Assay
Device3 Assay
Experiment
Experiment Overview
■Week 0(8.9-8.11)
■ 9th Aug
Preparation of culture plates with Ampicillin
■ 10th Aug
Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate
■ 11th Aug
Confirmation of the E.coli growth
Cultivation of the E.coli on LB culture
■1st week (8.13 - 8.19)
Make Device3→more
■2nd week (8.20 - 8.26)
Make Device1→more
■3rd week (8.27 - 9. 2)
Make Device1 and Make Device3→more
■4th week (9. 3 - 9. 9)
Make all Device→more
■5th week (9.10 - 9.16)
Make all Device→more
■6th week (9.17 - 9.23)
Make all Device→more
■7th week (9.24 - 9.30)
Assay→more and Assay protocol
