Team:UC Chile/Results/Vs

From 2012.igem.org

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<h1>sfGFP vs mRFP1</h1>
<h1>sfGFP vs mRFP1</h1>
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Improvement of an existing BioBrick
 
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Our chosen target was Biobrick J04450 which is a RFP Coding Device (link: http://partsregistry.org/Part:BBa_J04450). Its usage as a cloning tool has increased in time due to the easy detection of transformed colonies to the naked eye. Nonetheless, the advancement of automatic measurement systems (and yada yada) opens up a whole new range of applications in which markers are detected by highly sensitive machines and not by human observers. In these cases mRFP does not provide a significant advantage over other marker systems. In fact, it could be detrimental for the efficiency of the experiment (?)
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<font size="4">Improvement of an existing BioBrick</font>
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Our chosen target was Biobrick J04450 which is a mRFP Coding Device (link: http://partsregistry.org/Part:BBa_J04450). Its usage as a cloning tool has increased in time due to the easy detection of transformed colonies to the naked eye. Nonetheless, the advancement of automatic measurement systems (and yada yada) opens up a whole new range of applications in which markers are detected by highly sensitive machines and not by human observers. In these cases mRFP does not provide a significant advantage over other marker systems. In fact, it could diminish the efficiency of the experiment.
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To demonstrate the improvement of the biobrick, we designed and run the following experiments:
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To demonstrate the improvement of the BioBrick, we designed and run the following experiments:
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Experiment : Culture growth
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<font size="3">Experiment : Culture growth</font>
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Three flasks were inoculated in equal concentrations with: psB4K5-J04450, psB4K5 – ours and wildtype TOP 10 cells respectively. Inoculum concentrations were checked by culture OD measurement at 600 nm to normalize the amount of bacteria that would be initially present.  
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Three flasks were inoculated in equal concentrations with: psB4K5-J04450, psB4K5 – K743017 and wildtype TOP 10 cells respectively. Inoculum concentrations were checked by culture OD measurement at 600 nm to normalize the amount of bacteria that would be initially present.  
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Then, OD measurements at 600 nm, 584 nm and 492 nm were reported every 30 minutes for each of the flasks. 584 nm and 492 nm are the peak absortion wavelengths of mRFP1 and sfGFP respectively.  
Then, OD measurements at 600 nm, 584 nm and 492 nm were reported every 30 minutes for each of the flasks. 584 nm and 492 nm are the peak absortion wavelengths of mRFP1 and sfGFP respectively.  
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Results 1. Growth rate
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Results 1. Growth rate at 600 nm
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Experimental data was adjusted in order to….
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Waiting for results ☺
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Experimental data was adjusted  
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eq
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curve
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Results 2.
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Results 2 Growth rate at 730 nm
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Experimental data was adjusted in order to….
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Waiting for results ☺
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Experimental data was adjusted
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eq
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curve
References
References
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Results 3.
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Revision as of 17:52, 26 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012