Team:UC Chile/Bactomithril

Contents 1 Overview 2 Introduction 3 Construct: a novel secretion system 4 Notebook 5 Results 6 Future Prospects: The construction of the first spider silk biobrick 7 References

Overview

Spider dragline silk is an exceptionally strong and elastic fiber and is an ideal material for numerous biomedical and industrial applications. In this secondary project we developed a novel secretion system that allows to export proteins to the extracellular medium and tried to add the first spider-silk biobrick to the Registry. This biobrick would have the synthetic silk gene ADF-3 from the orb weaving spider Araneus diadematus. Nevertheless, due to the repetitive nature of the spider silk monomer we could not finish this biobrick for the Regional Jamboree (despite several attempts and strategies).

Animation describing the first secretion system

Click here to open this animation from the begining in a new tab

Introduction

Spider dragline silk is an exceptionally strong and elastic biomaterial and one of the strongest natural fibers known. It is five times stronger by weight than steel and three times tougher than the top quality man-made fiber Kevlar [1]. That is why it is referred to as “biosteel”. Furthermore, this material is biocompatible, biodegradable and has potential for processing in aqueous solution under ambient conditions.

These properties make the spider silk an ideal material for numerous industrial applications such as bullet-proof vests, parachutes, seat belts and composite materials in aircraft, and a promising substance for biomedical applications such as drug delivery systems and scaffolds for tissue engineering.

The excellent mechanical properties and low immunogenicity of spider webs have fascinated the man for thousands of years, but unfortunately large scale farming of spiders is not convenient because of the highly territorial and aggressive character of these arthropods. Synthetic spider silk production is a promising way to make use of this exceptional biomaterial.

Construct: a novel secretion system

Working Plasmid ([http://partsregistry.org/Part:BBa_K743011 BBa_K43011])

This is an intermediate plasmid that we used to build the GFP Reporter Plasmid an the Protease Producer Plasmid.

GFP Reporter Plasmid ([http://partsregistry.org/Part:BBa_K743012 BBa_K43012])

The description of the mechanism of this construct is explained in the animation below.

Protease Producer Plasmid ([http://partsregistry.org/Part:BBa_K743013 BBa_K43013])

This construct induces the expression of the HIV protease.

Notebook

In this section you will find the complete description of our work in this project.

Results

You can see detailed informarion of our results in this section. Nevertheless, we can briefly summarize the following: we know that sfGFP expresses in our e. coli, but we don’t know if it is attached to the external side of the membrane, or if the HIV-1 protease works as expected. As this secretion system seems to be failing, we chose to try other alternatives.

Future Prospects: The construction of the first spider silk biobrick

We tried with a lot of patience (in SEVERAL attempts with many different strategies) to make a biobrick with the spider silk monomer ADF-3 or the sequence of DNA corresponding to ADF-3 and tags necessary to identify and export the protein with the Type 3 Secretion System of Salmonella (SPI 2). The original sequences are described in

Widmaier, D. M., Tullman-Ercek, D., Mirsky, E. a, Hill, R., Govindarajan, S., Minshull, J., & Voigt, C. a. (2009). Engineering the Salmonella type III secretion system to export spider silk monomers. Molecular systems biology, 5(309), 309. doi:10.1038/msb.2009.62

This sequence was kindly sent by Dr. Christopher Voigt to us.

A detailed description of this work is in the notebook section. After tons of hours of hard work, when we finally obtained PCR amplified bands of the correct size, the digestion tests were positive, colony PCR were succesful and Gibson Assemblies seemed to work, we sent our final construct for sequencing and the results did not match with our designs. Probably this is due to the hihgly repetitive nature of the spider silk monomers.

References

In this section you will find references about production and modelling of recombinant spider silk proteins.

Recommended literature

Teulé, F., Cooper, A. R., Furin, W. a, Bittencourt, D., Rech, E. L., Brooks, A., & Lewis, R. V. (2009). A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning. Nature protocols, 4(3), 341-55. doi:10.1038/nprot.2008.250

Widhe, M., Johansson, J., Hedhammar, M., & Rising, A. (2012). Invited review current progress and limitations of spider silk for biomedical applications. Biopolymers, 97(6), 468-78. doi:10.1002/bip.21715

Widmaier, D. M., Tullman-Ercek, D., Mirsky, E. a, Hill, R., Govindarajan, S., Minshull, J., & Voigt, C. a. (2009). Engineering the Salmonella type III secretion system to export spider silk monomers. Molecular systems biology, 5(309), 309. doi:10.1038/msb.2009.62

Xia, X.-X., Qian, Z.-G., Ki, C. S., Park, Y. H., Kaplan, D. L., & Lee, S. Y. (2010). Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber. Proceedings of the National Academy of Sciences of the United States of America, 107(32), 14059-63. doi:10.1073/pnas.1003366107

Complementary literature

An, B., Hinman, M. B., Holland, G. P., Yarger, J. L., & Lewis, R. V. (2011). Inducing β-sheets formation in synthetic spider silk fibers by aqueous post-spin stretching. Biomacromolecules, 12(6), 2375-81. doi:10.1021/bm200463e

Askarieh, G., Hedhammar, M., Nordling, K., Saenz, A., Casals, C., Rising, A., Johansson, J., et al. (2010). Self-assembly of spider silk proteins is controlled by a pH-sensitive relay. Nature, 465(7295), 236-8. doi:10.1038/nature08962

Ayoub, N. a, Garb, J. E., Tinghitella, R. M., Collin, M. a, & Hayashi, C. Y. (2007). Blueprint for a high-performance biomaterial: full-length spider dragline silk genes. PloS one, 2(6), e514. doi:10.1371/journal.pone.0000514

Bauer, F., & Scheibel, T. (2012). Artificial egg stalks made of a recombinantly produced lacewing silk protein. Angewandte Chemie (International ed. in English), 51(26), 6521-4. doi:10.1002/anie.201200591

Bayley, H. (1998). Puri ® cation and characterization of recombinant spider silk expressed in Escherichia coli. Cell, 1, 31-38.

Bogush, V. G., Sokolova, O. S., Davydova, L. I., Klinov, D. V., Sidoruk, K. V., Esipova, N. G., Neretina, T. V., et al. (2009). A novel model system for design of biomaterials based on recombinant analogs of spider silk proteins. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 4(1), 17-27. doi:10.1007/s11481-008-9129-z

Dams-Kozlowska, H., Majer, A., Tomasiewicz, P., Lozinska, J., Kaplan, D. L., & Mackiewicz, A. (2012). Purification and cytotoxicity of tag-free bioengineered spider silk proteins. Journal of biomedical materials research. Part A, 1-9. doi:10.1002/jbm.a.34353

Das, R., & Baker, D. (2008). Macromolecular modeling with rosetta. Annual review of biochemistry, 77, 363-82. doi:10.1146/annurev.biochem.77.062906.171838

Kenney, J. M., Knight, D., Wise, M. J., & Vollrath, F. (2002). Amyloidogenic nature of spider silk. European Journal of Biochemistry, 269(16), 4159-4163. doi:10.1046/j.1432-1033.2002.03112.x

Keten, S., & Buehler, M. J. (2010). Nanostructure and molecular mechanics of spider dragline silk protein assemblies. Journal of the Royal Society, Interface / the Royal Society, 7(53), 1709-21. doi:10.1098/rsif.2010.0149

Lazaris, A., Arcidiacono, S., Huang, Y., Zhou, J.-F., Duguay, F., Chretien, N., Welsh, E. a, et al. (2002). Spider silk fibers spun from soluble recombinant silk produced in mammalian cells. Science (New York, N.Y.), 295(5554), 472-6. doi:10.1126/science.1065780

Moisenovich, M. M., Pustovalova, O. L., Arhipova, a Y., Vasiljeva, T. V., Sokolova, O. S., Bogush, V. G., Debabov, V. G., et al. (2011). In vitro and in vivo biocompatibility studies of a recombinant analogue of spidroin 1 scaffolds. Journal of biomedical materials research. Part A, 96(1), 125-31. doi:10.1002/jbm.a.32968

Rising, A., Widhe, M., Johansson, J., & Hedhammar, M. (2011). Spider silk proteins: recent advances in recombinant production, structure-function relationships and biomedical applications. Cellular and molecular life sciences : CMLS, 68(2), 169-84. doi:10.1007/s00018-010-0462-z

Tobar, J. a, Carreño, L. J., Bueno, S. M., González, P. a, Mora, J. E., Quezada, S. a, & Kalergis, A. M. (2006). Virulent Salmonella enterica serovar typhimurium evades adaptive immunity by preventing dendritic cells from activating T cells. Infection and immunity, 74(11), 6438-48. doi:10.1128/IAI.00063-06

Vollrath, F. (1999). Biology of spider silk. International journal of biological macromolecules, 24(2-3), 81-8. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10342751

Vollrath, F., & Knight, D. P. (2001). Liquid crystalline spinning of spider silk. Nature, 410(6828), 541-8. doi:10.1038/35069000

Winkler, S., Szela, S., Avtges, P., Valluzzi, R., Kirschner, D. a, & Kaplan, D. (1999). Designing recombinant spider silk proteins to control assembly. International journal of biological macromolecules, 24(2-3), 265-70. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10342773