Team:UC Chile/Labook/march

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<ul>Lab book:<br>  
<ul>Lab book:<br>  
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<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li>
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<li><a href="https://2012.igem.org/Team:UC_Chile/Labook/march">March</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/april">April</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/may">May</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/june">June</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/july">July</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/august">August</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/september">September</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Labook/october">October</a></li>
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<p><font size= "5">March</font></p>
<p><font size= "5">March</font></p>
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<font size="4">Monthly Summary:</font>
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We started with the design of constructs to express RFP under circadian promoters (psbAB and psBA2) and their respective primers. The amplification of the parts needed for the constructs are obtained by PCR. There have been several problems related to the amplification of Kanr. The PCR has been repeated with high frequency with no significant results.
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As we decided to do two different projects, the team was divided into subgroups and each person has specific tasks.  These are mainly related to dry and wet lab areas.
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<font size="4">Constructs and primers design</font>                                           
<font size="4">Constructs and primers design</font>                                           
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First constructs. Check: https://2012.igem.org/Team:UC_Chile2/Cyanolux/Biobricks                                                                     
First constructs. Check: https://2012.igem.org/Team:UC_Chile2/Cyanolux/Biobricks                                                                     
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Primer sequences                                                                   
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  ----------  ---------------  -----------------                                       
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Strategy for integration construct:                                       
Strategy for integration construct:                                       
Part K292003 is not in the kit. It will be assembled  from P1003+B0012+B0014  
Part K292003 is not in the kit. It will be assembled  from P1003+B0012+B0014  
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<font size="4">Getting parts ready</font>  
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<font size="4">Getting parts ready</font>
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Plates prepared
Plates prepared
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PCR for pPMQAK1, psbAB, RFP.<br>     
PCR for pPMQAK1, psbAB, RFP.<br>     
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<font size="4">Work plan</font>                                                    
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<font size="4">Work plan</font>
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Labour division: Bactomithril {Max, Emilia,Ulises, Claudia, Bryon}<br>
Labour division: Bactomithril {Max, Emilia,Ulises, Claudia, Bryon}<br>
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Meetings with Dr. Gutiérrez every two weeks to check work done.                             
Meetings with Dr. Gutiérrez every two weeks to check work done.                             
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<font size="4">Monthly Summary:</font>
 
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We started with the design of constructs to express RFP under circadian promoters (psbAB and psBA2) and their respective primers. The amplification of the parts needed for the constructs are obtained by PCR. There have been several problems related to the amplification of Kanr. The PCR has been repeated with high frequency with no significant results.
 
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As we decided to do two different projects, the team was divided into subgroups and each person has specific tasks.  These are mainly related to dry and wet lab areas.
 
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Latest revision as of 19:17, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012