Team:UC Chile/Cyanolux/Future

From 2012.igem.org

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<h1>Promoters Redesigned</h1>
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<h1>Project status</h1>
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We [https://2012.igem.org/Team:UC_Chile/Results/LuxBrick#N-decanal_Effect_Characterization_-_.28BBa_K325909_and_BBa_K325905.29 have seen] that even if induced with substrate, our luciferase constructs don´t show luminescence, though the constructs´ sequences are verified and well characterized luciferases do show light emition.
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Our advisors in cyanobacteria have told us that sometimes promoter sequences can be placed several hundred of bases upstream the +1 site, even inside protein coding sequences.
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That´s why we are designing new versions of our chosen promoters. Now we have changed the promoter construction methodology in terms of the lenght of the upstream sequence considered.
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Instead of considering just 150-200bp, up to 1000bp will be amplified from Synechocystis genome.
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<h1>Transcriptional verification</h1>
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Currently, we have been able to detect light-emission from our <i>Synechocystis</i> cells, however we still need to analyze circadian expression of the genes. Furthermore, we still have not been able to assemble the substrate production enzymes to the susceptibility plasmid. We hope that with out new strategy we will be successful. Once we are able to integrate the substrate production genes we may be able to assess the desired behavior of our system and analyze its impact on the chassis.  
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Even if our promoters are o.k, colony PCRs can´t tell us wether the transcriptional machinery of our cyanobacteria is recognizing our constructs.
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We are designig new primers to make  RT-PCRs that unmistakably verify transcription of the Lux genes at the specified hours.
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<h1>Circadian expression characterization</h1>
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After having the complete system we will have to see if we can achieve visible lighting with <i>Luxilla</i>. If not, we will try optimizing codon usage for Synechocystis and/or changing promoters.
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We have already built part [http://partsregistry.org/Part:BBa_K743018 BBa_K743018] to characterize differential expression of sfGFP at different moments of the day.
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Other promoters (new ones or those already built) will be placed by means of Gibson assembly controlling the expression of this reporter.
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<h1>New Biosafety strategies</h1>
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<h1>Biological lighting</h1>
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We have already assembled part [http://partsregistry.org/Part:BBa_K743010 psb1C3_IntS] wich has a double goal: to insert LuxCDEG genes into Synechocystis and to confere a copper susceptibility that makes this cells unable to thrive in the environment.
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We are also designing primers to built our [https://2012.igem.org/File:UC_Chile-Animacion-mE-gen.gif new biosafety system ] based on mE genes and MgSO4, which will be tested as soon as it is ready.
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<h1>Biolamp development</h1>
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Energy requirements keep on increasing. Resource consumption and dependence on fossil fuels has led modern society to use crops for fuel production. (<b>This sounds awfull</b>)
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When all the lux genes are inserted into Synechocystis under the promoters recomended by our [https://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling modelling] we will make a new bioluminescense assay in the luminometer to test if our strain is ready to be grown in the biolamp.
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The [https://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp biolamp prototype] will be built so bacteria can be placed inside it.
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Sustainability is needed desperately, and we believe that <i>Luxilla</i> might bring some aid in that aspect.
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Finally we will characterize the sustainability of wt Synechocystis and our recombinant strain inside the biolamp.
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We have thought about real practical applications of <i>Luxilla</i> on passive lighting, specially in areas of transit like corridors or sidewalks.  
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<h1>Context in Synbio</h1>
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Since we started developing this project we have realized the importance of biological context, specially when functionality is a desired aspect of the behavioral output of the system. While in one chassis a Biobrick may provide a novel function, in another it may become a practical application for society.
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We have seen first hand the difficulties associated with this aspect of current progress in SynBio. (JAJAJ acabo de escribir SyneBIO). By lacking standarized and proven methodologies and relying on previous uncharacterized work. We urge the Synbio community to address this aspect by encouraging the use and characterization of different chassis. It is of utmost importance that BioBrick designed for the chassis must be characterized in the chassis or they may prove fallible. The same can be said about failed experiences with BioBricks that are not reported.
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<b>VIDEO ACA?</b>
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Revision as of 10:36, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012