Team:UC Chile/Cyano/Notepad/week9
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- | <ul>Quest to destroy the ring : | + | <ul>Quest to destroy the ring : |
- | <li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week1"> | + | |
- | <li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week6"> | + | <li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad">0</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week1">1</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week2">2</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week3">3</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week4">4</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week5">5</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week6">6</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week7">7</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week8">8</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week9">9</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week10">10</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week11">11</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week12">12</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week13">13</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week14">14</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week15">15</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week16">16</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week17">17</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week18">18</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week19">19</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week20">20</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week21">21</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week22">22</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week23">23</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week24">24</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week25">25</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week26">26</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week27">27</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week28">28</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week29">29</a></li><li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week30">30</a></li> |
- | <li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week7"> | + | |
- | <li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week8"> | + | |
- | <li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week9"> | + | |
- | <li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week10"> | + | |
- | <li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week11"> | + | |
- | <li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week12"> | + | |
- | <li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week13"> | + | |
- | <li><a href="https://2012.igem.org/Team:UC_Chile/Cyano/Notepad/week14"> | + | |
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+ | <p><font size="5">April 30 - May 6</font></p> | ||
+ | <br> | ||
+ | |||
+ | <b>Monday</b> | ||
Did a massive PCR run. We amplified every part of Construct 1 using as template old PCR purifications and PCR products. We'll build C1 from scratch. Hoping everything works out well this time. | Did a massive PCR run. We amplified every part of Construct 1 using as template old PCR purifications and PCR products. We'll build C1 from scratch. Hoping everything works out well this time. | ||
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Muchos años después, al frente del pelotón de fusilamiento, el coronel Aureliano Buendía había de recordar aquella remota tarde en que su padre lo llevó a conocer el hielo. | Muchos años después, al frente del pelotón de fusilamiento, el coronel Aureliano Buendía había de recordar aquella remota tarde en que su padre lo llevó a conocer el hielo. | ||
+ | These are the first lines of A hundred years of solitude by G.G. Márquez :) | ||
- | Tuesday | + | |
+ | <b>Tuesday</b> | ||
We decided to transform e. coli with the expression plasmid pPMQAK1. Also, as the mentioned plasmid contains ccdb toxin, we took it out by a digestion/ligation procedure and transformed again. | We decided to transform e. coli with the expression plasmid pPMQAK1. Also, as the mentioned plasmid contains ccdb toxin, we took it out by a digestion/ligation procedure and transformed again. | ||
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Existential Question: WHAT DO YOU DO WHEN YOU HAVE A GREMLIN PLAGUE IN THE LABORATORY?? | Existential Question: WHAT DO YOU DO WHEN YOU HAVE A GREMLIN PLAGUE IN THE LABORATORY?? | ||
- | Got it!! | + | Got it!! Use a protocol! |
- | + | Need to adapt one ;) | |
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- | + | <b>Wednesday</b> | |
- | Wednesday | + | |
Talked with one of our advisors today. He gave us interesting input on our failed PCR's. | Talked with one of our advisors today. He gave us interesting input on our failed PCR's. | ||
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Checked PCR runs. KanR didn't have the strenght we'd have liked, but still we used it. All parts were assembled by Gibson. Also, every part has its neg. control. After Gibson, we transformed coli in kan, chlor and both resistance plates. | Checked PCR runs. KanR didn't have the strenght we'd have liked, but still we used it. All parts were assembled by Gibson. Also, every part has its neg. control. After Gibson, we transformed coli in kan, chlor and both resistance plates. | ||
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- | Friday | + | <b>Friday</b> |
Happy Star Wars day!!!! May the fourth be with you :) | Happy Star Wars day!!!! May the fourth be with you :) | ||
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+ | {{UC_Chilefooter}} |
Latest revision as of 00:19, 23 September 2012