Team:UC Chile/Cyano/Notepad/week9
From 2012.igem.org
(Difference between revisions)
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- | + | <b>Monday</b> | |
- | Monday | + | |
Did a massive PCR run. We amplified every part of Construct 1 using as template old PCR purifications and PCR products. We'll build C1 from scratch. Hoping everything works out well this time. | Did a massive PCR run. We amplified every part of Construct 1 using as template old PCR purifications and PCR products. We'll build C1 from scratch. Hoping everything works out well this time. | ||
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- | Tuesday | + | <b>Tuesday</b> |
We decided to transform e. coli with the expression plasmid pPMQAK1. Also, as the mentioned plasmid contains ccdb toxin, we took it out by a digestion/ligation procedure and transformed again. | We decided to transform e. coli with the expression plasmid pPMQAK1. Also, as the mentioned plasmid contains ccdb toxin, we took it out by a digestion/ligation procedure and transformed again. | ||
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- | Wednesday | + | <b>Wednesday</b> |
Talked with one of our advisors today. He gave us interesting input on our failed PCR's. | Talked with one of our advisors today. He gave us interesting input on our failed PCR's. | ||
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Checked PCR runs. KanR didn't have the strenght we'd have liked, but still we used it. All parts were assembled by Gibson. Also, every part has its neg. control. After Gibson, we transformed coli in kan, chlor and both resistance plates. | Checked PCR runs. KanR didn't have the strenght we'd have liked, but still we used it. All parts were assembled by Gibson. Also, every part has its neg. control. After Gibson, we transformed coli in kan, chlor and both resistance plates. | ||
- | |||
- | Friday | + | <b>Friday</b> |
Happy Star Wars day!!!! May the fourth be with you :) | Happy Star Wars day!!!! May the fourth be with you :) | ||
{{UC_Chilefooter}} | {{UC_Chilefooter}} |
Revision as of 00:18, 23 September 2012