Team:UC Chile/Accomplishments
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<br>✓ At least one new submitted and well-characterized standard BioBrick Part or Device. A new application of and outstanding documentation (quantitative data showing the Part’s/ Device’s function) of a previously existing BioBrick part in the “Experience” section of that BioBrick’s Registry entry also counts. | <br>✓ At least one new submitted and well-characterized standard BioBrick Part or Device. A new application of and outstanding documentation (quantitative data showing the Part’s/ Device’s function) of a previously existing BioBrick part in the “Experience” section of that BioBrick’s Registry entry also counts. | ||
- | <i>We submitted parts [http://partsregistry.org/Part:BBa_K743000 BBa_K743000] and [http://partsregistry.org/Part:BBa_K743001 BBa_K743001] | + | <i>We submitted parts [http://partsregistry.org/Part:BBa_K743000 BBa_K743000] and [http://partsregistry.org/Part:BBa_K743001 BBa_K743001] which were verified by digestion and sequencing. The functioning of these parts was extensively proved by our successful transformation of synechocystis that is well documented in our [https://2012.igem.org/Team:UC_Chile/Cyanolux/Results results]. |
We also characterized the functioning of KanR resistance genes [http://partsregistry.org/Part:BBa_P1003 P1003] and [http://partsregistry.org/Part:BBa_P1007 P1007] in Synechocystis PCC6803. | We also characterized the functioning of KanR resistance genes [http://partsregistry.org/Part:BBa_P1003 P1003] and [http://partsregistry.org/Part:BBa_P1007 P1007] in Synechocystis PCC6803. | ||
- | Lastly, we quantitatively characterized the [http://partsregistry.org/Part:BBa_K325909 Luxbrick] in different temperature, | + | Lastly, we quantitatively characterized the [http://partsregistry.org/Part:BBa_K325909 Luxbrick] in different temperature, O.D, glucose concentration and growth fase conditions.</i> |
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<i>Parts [http://partsregistry.org/Part:BBa_K743000 BBa_K743000] and [http://partsregistry.org/Part:BBa_K743001 BBa_K743001] worked as expected in Synechocystis double homologous recombination transformations. | <i>Parts [http://partsregistry.org/Part:BBa_K743000 BBa_K743000] and [http://partsregistry.org/Part:BBa_K743001 BBa_K743001] worked as expected in Synechocystis double homologous recombination transformations. | ||
Part [http://partsregistry.org/Part:BBa_K743017 BBa_K743017] showed inducible expression as planned. | Part [http://partsregistry.org/Part:BBa_K743017 BBa_K743017] showed inducible expression as planned. | ||
- | Device [http://partsregistry.org/Part:BBa_K743006 BBa_K743006] showed the right results, i.e was | + | Device [http://partsregistry.org/Part:BBa_K743006 BBa_K743006] showed the right results, i.e was useful for transformation and selection in Synechocystis.</i> |
- | <br>✓Characterize the operation of at least one new BioBrick Part or Device and enter this information in the “Main Page” section of that Part’s/Device’s Registry entry | + | <br>✓Characterize the operation of at least one new BioBrick Part or Device and enter this information in the “Main Page” section of that Part’s/Device’s Registry entry. |
- | <i>Device [http://partsregistry.org/Part:BBa_K743006 BBa_K743006] undergoes double homologous recombination in Synechocystis PCC6803 and | + | <i>Device [http://partsregistry.org/Part:BBa_K743006 BBa_K743006] undergoes double homologous recombination in Synechocystis PCC6803 and confers kanamycin resistance. Other sequences can be added to it and its effectiveness remains as shown in [http://partsregistry.org/Part:BBa_K743005 BBa_K743005]</i> |
Revision as of 05:52, 26 September 2012