Team:UANL Mty-Mexico/Notebook

From 2012.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 6: Line 6:
<p><b>Fusion proteins</b></p>
<p><b>Fusion proteins</b></p>
 +
<br>
<p>We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.</p>
<p>We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.</p>
Line 23: Line 24:
<tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/5/5f/Assembly_21-2.png/569px-Assembly_21-2.png" style="width:450px;"></td></tr>
<tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/5/5f/Assembly_21-2.png/569px-Assembly_21-2.png" style="width:450px;"></td></tr>
</table>
</table>
 +
 +
<p><br></p>
 +
 +
For the rest of the constructs Standard Assembly 10 was used. Check our results on the "wet lab" tab.
Line 32: Line 37:
  <div class="sidebar2">
  <div class="sidebar2">
     <ul class="nav">
     <ul class="nav">
-
<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook"><li><b>>>Notebook</b></a></li>
+
<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook"><li><b>>>Cloning Strategy</b></a></li>
<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/wetlab"><li>Wetlab</a></li>
<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/wetlab"><li>Wetlab</a></li>
<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/protocols"><li>Protocols</a></li>
<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/protocols"><li>Protocols</a></li>

Latest revision as of 04:06, 27 September 2012

iGEM UANL 2012


Cloning Strategy


Fusion proteins


We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.




Figure 1. Cloning strategy for contructions of fusion proteins.


For the rest of the constructs Standard Assembly 10 was used. Check our results on the "wet lab" tab.



logo

Retrieved from "http://2012.igem.org/Team:UANL_Mty-Mexico/Notebook"