Team:TU Darmstadt/Protocols/Bacterial transformation

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== Bacterial transformation ==
== Bacterial transformation ==
There a several methods of bacterial transformation, the process of inserting DNA into a host, the most common are:
There a several methods of bacterial transformation, the process of inserting DNA into a host, the most common are:
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation]'''and'''
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation]
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and  
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heatshock Transformation]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heatshock Transformation]
The transformation of ''E. coli'' with plasmid DNA according to those methods do require [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Chemically_competent_cells chemically]/[https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells electro]-competent cells.
The transformation of ''E. coli'' with plasmid DNA according to those methods do require [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Chemically_competent_cells chemically]/[https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells electro]-competent cells.

Latest revision as of 18:41, 26 September 2012

Bacterial transformation

There a several methods of bacterial transformation, the process of inserting DNA into a host, the most common are:

and

The transformation of E. coli with plasmid DNA according to those methods do require chemically/electro-competent cells.

Application

References

  • Praktikumsskript Biotechnologie 2012 TU Darmstadt