Team:TU Darmstadt/Protocols/Heat Shock Transformation

From 2012.igem.org

Heat Shock Transformation

About

Heat shock transformation is a method of bacterial transformation.

Procedure

  1. Defrost stocks of competent cells (100 µL in 1.5 ml Eppendorf tube) on ice
  2. Add the DNA and incubate the suspension for 5 minutes on ice
  3. Heat shock is done by incubating the cells for 1 minute at 42°C
  4. Add 1 mL of DYT medium and incubate for 1 hour at 37°C in order to obtain antibiotic resistance
  5. Finally the cells are ready to be crossed out
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