Microcapsule degradation

We implemented microencapsulation of engineered human cells and designed the system for degradation of alginate microcapsules.

Engineered HEK293T cells were succesfully incorporated into alginate microcapsules and extended time viability of the encapsulated cells was demonstrated.

Secretory alginate lyases from Pseudomoalteromonas elyakovii and from Pseudomonas aeruginosa were cloned by replacing the signal peptide of the alginate lyase with the eukaryotic signal peptide.

Alginate lyases were succesfully produced and secreted from HEK293T cells.

Degradation of alginate beads was demonstrated by alginate lyase from Sphingobacterium multivorum.

References

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