Team:Osaka/week4
From 2012.igem.org
(Difference between revisions)
Line 42: | Line 42: | ||
# Restriction digests | # Restriction digests | ||
# Electrophoresis:155,156,1-1G,1-3A,1-5A,1-7A | # Electrophoresis:155,156,1-1G,1-3A,1-5A,1-7A | ||
- | #* | + | #*155,156 × |
# Transformaton of ligation products into DH5α <i>E.coli</i>:1-1G,5-23O,1-5A,1-7A | # Transformaton of ligation products into DH5α <i>E.coli</i>:1-1G,5-23O,1-5A,1-7A | ||
[[Team:Osaka/Notebook|Back to Notebook]] | [[Team:Osaka/Notebook|Back to Notebook]] |
Revision as of 01:50, 27 August 2012
August 19 (Sun)
- Colony check
- Transformed cells didn't grow on plates
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 20 (Mon)
- Colony check
- Transformed cells didn't grow on plates
- Transformation of plasmid DNA into DH5α E.coli
- test the efficiency of competent cells
August 21 (Tue)
- Colony check
- successfully transformed
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 22 (Wed)
- Transfer to liquid culture
- turned red → retrial
August 23 (Thu)
- Restriction digest
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 24 (Fri)
We had an interview about our project. #gemFM
- Transfer to liquid culture: 155,156
August 25 (Sat)
- Miniprep: 155,156
- Restriction digests
- Electrophoresis:155,156,1-1G,1-3A,1-5A,1-7A
- 155,156 ×
- Transformaton of ligation products into DH5α E.coli:1-1G,5-23O,1-5A,1-7A