Team:Osaka/week4

August 19 (Sun)

1. Colony check
   • Transformed cells didn't grow on plates
2. Ligation:155,156
3. Transformaton of ligation products into DH5α E.coli:155,156

August 20 (Mon)

1. Colony check
   • Transformed cells didn't grow on plates
2. Transformation of plasmid DNA into DH5α E.coli
   • test the efficiency of competent cells

August 21 (Tue)

1. Colony check
   • successfully transformed
2. Ligation:155,156
3. Transformaton of ligation products into DH5α E.coli:155,156

August 22 (Wed)

1. Transfer to liquid culture
   • turned red → retrial

August 23 (Thu)

1. Restriction digest
2. Ligation:155,156
3. Transformaton of ligation products into DH5α E.coli:155,156

August 24 (Fri)

We had an interview about our project. #gemFM

1. Transfer to liquid culture: 155,156

August 25 (Sat)

1. Miniprep: 155,156
2. Restriction digests
3. Electrophoresis:155,156,1-1G,1-3A,1-5A,1-7A
   • 155,156 ×
4. Transformaton of ligation products into DH5α E.coli:1-1G,5-23O,1-5A,1-7A

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