Team:NTNU Trondheim/Stocks

From 2012.igem.org

(Difference between revisions)
(→Isolated DNA)
m (→Isolated DNA)
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! Name/abbreviation ||  Date stored ||  Description  || Concentration|| Backbone
! Name/abbreviation ||  Date stored ||  Description  || Concentration|| Backbone
|-
|-
-
|LuxR+Term||16.7||BBa_C0062 + BBa_B0015 ||37,0 ||pSB1A2
+
|LuxR+Term||16.7||BBa_C0062 + BBa_B0015 ||37,0 ||pSB1AK3
|-
|-
-
|LuxI+Term||16.7||BBa_C0061 + BBa_B0015 ||37,6 ||pSB1A2
+
|LuxI+Term||16.7||BBa_C0061 + BBa_B0015 ||37,6 ||pSB1AK3
|-
|-
|C79||27.06||<partinfo>BBa_C0079</partinfo>||70,8||pSB2K3
|C79||27.06||<partinfo>BBa_C0079</partinfo>||70,8||pSB2K3
Line 42: Line 42:
|GFP+Term||11.07||BBa_K082003 + BBa_B0015 || ||pSB1AK3
|GFP+Term||11.07||BBa_K082003 + BBa_B0015 || ||pSB1AK3
|-
|-
-
|CFP+Term||11.07||BBa_E0020 + BBa_B0015 || ||pSB1A2
+
|CFP+Term||11.07||BBa_E0020 + BBa_B0015 || ||pSB1AK3
|-
|-
|LI (BBa_K092400)||?||<partinfo>BBa_K092400</partinfo>|| ||pSB1A2
|LI (BBa_K092400)||?||<partinfo>BBa_K092400</partinfo>|| ||pSB1A2
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|VGB DNA||25.06||<partinfo>BBa_K561001</partinfo>|| ||pSB1C3
|VGB DNA||25.06||<partinfo>BBa_K561001</partinfo>|| ||pSB1C3
|-
|-
-
|RFP +Term||11.07||<partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> ||pSB2K3
+
|RFP +Term||11.07||<partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> ||pSB1AK3
|-
|-
|Constitutive promoter||22.06||<partinfo>BBa_J23119</partinfo> ||27,7||pSB1A2
|Constitutive promoter||22.06||<partinfo>BBa_J23119</partinfo> ||27,7||pSB1A2
Line 106: Line 106:
|Colicin||4.07||<partinfo>BBa_K150009</partinfo>||58,6||
|Colicin||4.07||<partinfo>BBa_K150009</partinfo>||58,6||
|-
|-
-
|VGB+RBS+YFP+DTT||26.07|| || ||pSB1A2
+
|VGB+RBS+YFP+DTT||26.07|| || ||pSB1AK3
|-
|-
-
|VHB+RBS+YFP+DTT||26.07|| || ||pSB1A2
+
|VHB+RBS+YFP+DTT||26.07|| || ||pSB1AK3
|-
|-
|EYFP+RBS+YFP+DTT||26.07|| || ||
|EYFP+RBS+YFP+DTT||26.07|| || ||

Revision as of 19:03, 28 July 2012

This page lists stored cultures and other samples:

See also Constructs page.

Resuspended DNA

(Dried DNA from distribution kit resuspended in dH2O)

Name/abbreviation Date stored Description Source Backbone
LR? <partinfo>BBa_C0062</partinfo>(RS)pSB1A2
Lysis22.06 BBa_K112808(RS)pSB1A2
C78?<partinfo>BBa_C0078</partinfo>(RS)
C79?<partinfo>BBa_C0079</partinfo>(RS)
RBS24.06<partinfo>BBa_B0034</partinfo>(RS)pSB1A2
LuxI?<partinfo>BBa_C0061</partinfo>(RS)
K+RBS?<partinfo>BBa_K081005</partinfo>(RS)pSB1A2

Isolated DNA

(Miniprep, gel extraction and PCR products)

Name/abbreviation Date stored Description Concentration Backbone
LuxR+Term16.7BBa_C0062 + BBa_B0015 37,0 pSB1AK3
LuxI+Term16.7BBa_C0061 + BBa_B0015 37,6 pSB1AK3
C7927.06<partinfo>BBa_C0079</partinfo>70,8pSB2K3
C7827.06<partinfo>BBa_C0078</partinfo>63,4pSB2K3
RBS27.06<partinfo>BBa_B0034</partinfo>30,2pSB1A2
GFP+Term11.07BBa_K082003 + BBa_B0015 pSB1AK3
CFP+Term11.07BBa_E0020 + BBa_B0015 pSB1AK3
LI (BBa_K092400)?<partinfo>BBa_K092400</partinfo> pSB1A2
YFP+Term11.07YFP+term (religated)?
DLD + RBS9.07BBa_K284003 + BBa_B0034 24,1
VHB + RBS9.07BBa_K258005 + BBa_B0034 48,3
Jen1 + RBS9.07BBa_K284002 + BBa_B0034 35,3
VGB + RBS9.07BBa_K561001 + BBa_B0034 40,0
LR?<partinfo>BBa_B0062</partinfo> 20,3
VGB promoter27.06<partinfo>BBa_K561001</partinfo>22,9pSB1C3
VGB DNA25.06<partinfo>BBa_K561001</partinfo> pSB1C3
RFP +Term11.07<partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> pSB1AK3
Constitutive promoter22.06<partinfo>BBa_J23119</partinfo> 27,7pSB1A2
DLD4.07<partinfo>BBa_K284003</partinfo>63,8BBa_J63010
K+RBS13.07<partinfo>BBa_K081005</partinfo>19,8pSB1A2
LuxR+HSL24.06<partinfo>BBa_R0062</partinfo> pSB1A2
pBAD+lysis9.7 23,6pSB1A3
DNA lysis24.06
D222.6BBa_K14515023,1pSB1A2
RBS6.07<partinfo>BBa_B0034</partinfo>44,5pSB1A2
LuxI6.07 38,6
VHB4.07<partinfo>BBa_K258005</partinfo>44,9pSB1A2
Jen4.07<partinfo>BBa_K284002</partinfo>102,1
luxR+HSL25.06 13,3
Lysis1 DNA24.06
GFP+DTT13.07BBa_K082003+BBa_B001541,1pSB1AK3
YFP+DTT13.07BBa_E0030+BBa_B001555,5pSB1AK3
RFP+DTT13.07BBa_E1010+BBa_B001540,9pSB2K3
CFP+DTT13.07BBa_E0020+BBa_B001547,7pSB1A2
pBAD B11.07<partinfo>Bba_K206000</partinfo>24,0pSB1A3
D122.06<partinfo>BBa_K091146</partinfo>19,8pSB1A2
pBAD B21.07<partinfo>Bba_K206000</partinfo>19pSB1A3
Colicin4.07<partinfo>BBa_K150009</partinfo>58,6
VGB+RBS+YFP+DTT26.07 pSB1AK3
VHB+RBS+YFP+DTT26.07 pSB1AK3
EYFP+RBS+YFP+DTT26.07
pTet GFP26.07<partinfo>BBa_I13522</partinfo> pSB1A2
RBS26.06<partinfo>BBa_B0034</partinfo>16,8pSB1A2
DTT Amp26.06<partinfo>BBa_B0015</partinfo>35,8pSB1AK3
DTT Kan26.06<partinfo>BBa_B0015</partinfo>26,5pSB1AK3
RFP K22.06BBa_E1010 pSB2K3
GFP AK22.06BBa_K082003 pSB1AK3
CFP A22.06BBa_E0020 pSB1A2
YFP AK22.06 BBa_E0030pSB1AK3

Glycerol stocks

(Cell cultures kept at -80 C)

Name/abbreviation Date stored Strain Plasmid Position
pTet mRFP (BBa_I13521)27.07DH5apSB1A2
pTet GFP (BBa_I13522)27.07DH5apSB1A2
EYFP + LVA (BBa_E0430)27.07DH5apSB1A2
RFP (<partinfo>BBa_E1010</partinfo>) 18.07 DH5a pSB2K3
VGB (<partinfo>BBa_K561001</partinfo>)18.07DH5apSB1C3
LuxR+HSL (<partinfo>BBa_R0062</partinfo>)16.07 DH5apSB1A2
Lysis (<partinfo>BBa_K112808</partinfo>)16.07 DH5apSB1A2
pBAD (<partinfo>Bba_K206000</partinfo>)16.07 DH5apSB1A3
LuxR (<partinfo>BBa_C0062</partinfo>)16.07 DH5apSB1A2
GFP (<partinfo>BBa_K082003</partinfo>)16.07 DH5apSB1AK3
pBAD+Lysis16.07 DH5apSB1A3
DTT (<partinfo>BBa_B0015</partinfo>17.07 DH5apSB1AK3
D2 (<partinfo>BBa_K145150</partinfo>)17.07 DH5apSB1A2
CFP (<partinfo>BBa_E0020</partinfo>)17.07 DH5apSB1A2
YFP (<partinfo>BBa_E0030</partinfo>)17.07 DH5apSB1AK3
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