Team:NTNU Trondheim/Stocks

From 2012.igem.org

This page lists stored cultures and other samples:

Dates are in the format dd.mm.

See also Constructs page.

Contents

Stored samples

Resuspended DNA

(Dried DNA from distribution kit resuspended in dH2O)

Name/abbreviation Date stored Description Source Backbone
LR? BBa_C0062(RS)pSB1A2
Lysis22.06 BBa_K112808(RS)pSB1A2
C78?BBa_C0078(RS)pSB2K3
C79?BBa_C0079(RS)pSB2K3
RBS24.06BBa_B0034(RS)pSB1A2
LuxI?BBa_C0061(RS)pSB1A2
K+RBS?BBa_K081005(RS)pSB1A2

Isolated DNA

(Miniprep, gel extraction and PCR products)
NOT included here: Digestion and ligation mixtures/products

Name/abbreviation Date stored Description Concentration Backbone
pTet GFP26.07BBa_I13522 pSB1A2
VGB+RBS+YFP+DTT26.07
VHB+RBS+YFP+DTT26.07
EYFP (RBS+ LVA- TERM)26.07BBa_E0430 pSB1A2
LuxR+Term16.7BBa_C0062 + BBa_B0015 37,0 pSB1AK3
LuxI+Term16.7BBa_C0061 + BBa_B001537,6 pSB1AK3
LI (BBa_K092400)?BBa_K092400 pSB1A2
GFP+DTT13.07BBa_K082003+BBa_B001541,1pSB1AK3
YFP+DTT13.07BBa_E0030+BBa_B001555,5pSB1AK3
RFP+DTT13.07BBa_E1010+BBa_B001540,9pSB1AK3
CFP+DTT13.07BBa_E0020+BBa_B001547,7pSB1AK3
K+RBS13.07BBa_K08100519,8pSB1A2
DLD + RBS09.07BBa_K284003 + BBa_B0034 24,1pSB1A2
VHB + RBS09.07BBa_K258005 + BBa_B0034 48,3pSB1A2
Jen1 + RBS09.07BBa_K284002 + BBa_B0034 35,3pSB1A2
VGB + RBS09.07BBa_K561001 + BBa_B0034 40,0pSB1A2
pBAD+lysis09.07 23,6pSB1A3
RBS06.07BBa_B003444,5pSB1A2
LuxI06.07BBa_C0061 38,6pSB1A2
Colicin04.07BBa_K15000958,6pSB1A2
VHB04.07BBa_K25800544,9pSB1A2
Jen104.07BBa_K284002102,1BBa_J63010
DLD04.07BBa_K28400363,8BBa_J63010
pBAD B101.07BBa_K20600024,0pSB1A3
pBAD B201.07BBa_K20600019pSB1A3
LR?BBa_C0062 20,3pSB1A2
VGB promoter27.06BBa_K56100122,9pSB1C3
C7927.06BBa_C007970,8pSB2K3
C7827.06BBa_C007863,4pSB2K3
RBS27.06BBa_B003430,2pSB1A2
RBS26.06BBa_B003416,8pSB1A2
DTT Amp26.06BBa_B001535,8pSB1AK3
DTT Kan26.06BBa_B001526,5pSB1AK3
VGB DNA25.06BBa_K561001 pSB1C3
luxR+HSL25.06BBa_R0062 13,3pSB1A2
Lysis1 DNA24.06BBa_K112808 pSB1A2
LuxR+HSL24.06BBa_R0062 pSB1A2
DNA lysis24.06BBa_K112808 pSB1A2
Constitutive promoter22.06BBa_J23119 27,7pSB1A2
D222.6BBa_K14515023,1pSB1A2
D122.06BBa_K09114619,8pSB1A2
RFP K22.06BBa_E1010 pSB2K3
GFP AK22.06BBa_K082003 pSB1AK3
CFP A22.06BBa_E0020 pSB1A2
YFP AK22.06 BBa_E0030 pSB1AK3

Restriction digests and ligation mixtures

(Inserts and backbones, DNA treated with enzymes)


Name/abbreviation Date stored Description Enzymes
YFP+Term (religation)11.07YFP+term (religated)?pSB1AK3
GFP+Term (religation)11.07BBa_K082003 + BBa_B0015 pSB1AK3
CFP+Term (religation)11.07BBa_E0020 + BBa_B0015 pSB1AK3
RFP +Term (religation)11.07BBa_E1010+BBa_B0015 pSB1AK3

Glycerol stocks

(Cell cultures kept at -80 C)

Name/abbreviation Date stored Strain Plasmid Position
K+RBS+RFP+DTT?DH5a?C1
K+RBS+GFP+DTT?DH5a?C2
RBS (BBa_B0034)?DH5a?C3
pTet mRFP (BBa_I13521)27.07DH5apSB1A2C6
pTet GFP (BBa_I13522)27.07DH5apSB1A2C5
EYFP (RBS+ LVA- TERM) (BBa_E0430)27.07DH5apSB1A2C4
RFP (BBa_E1010) 18.07 DH5a pSB2K3 B6
VGB (BBa_K561001)18.07DH5apSB1C3B5
LuxR+HSL (BBa_R0062)16.07 DH5apSB1A2A1
Lysis (BBa_K112808)16.07 DH5apSB1A2A3
pBAD (BBa_K206000)16.07 DH5apSB1A3A4
LuxR (BBa_C0062)16.07 DH5apSB1A2A2
GFP (BBa_K082003)16.07 DH5apSB1AK3A5
pBAD+Lysis16.07 DH5apSB1A3 A6
DTT (BBa_B001517.07 DH5apSB1AK3 B1
D2 (BBa_K145150)17.07 DH5apSB1A2 B4
CFP (BBa_E0020)17.07 DH5apSB1A2 B2
YFP (BBa_E0030)17.07 DH5apSB1AK3B3

PCR Products

Name Target Primers Template Status
pllDpllD promoter K-12Gel OK. Ligated and transformed, but unconfirmed.
ColicinColicin operon ?Gel good - strong band.


Agar plates

Name/abbreviation Date made Description AntibioticPlasmid
EYFP+RBS+Term24.07BBa_E0430Amp
A. vic. GFP25.07BBa_E0040Amp
Kolisin2.07BBa_K150009Amp
pTet GFP24.07BBa_I13522Amp
pTet mRFP22.07BBa_I13521Amp
psB1C3 religering27.07 CmpSB1C3
pllD in pSB1C327.07 CmpSB1C3
BBa_E101017.07 Kan
VGB17.07BBa_K561001Cm
CFP16.07 Amp
DTT16.07BBa_B0015Kan
YFP16.07 Amp
VHB+RBS+YFP+DTT24.07VHB test constructAmp
VGB+RBS+YFP+DTT24.07VGB test constructAmp
RBS backbone03.07 ? Amp
GFP + term (DTT)11.7 Amp
Jen102.07BBa_K284002Amp
BBa_B003422.7RBSAmp
K+RBS+GFP+DTT18.07 Amp
VHB02.07BBa_K258005Amp
LuxR15.07BBa_C0061Amp
pBAD + lysis15.07BBa_K206000+BBa_K112808Amp
Lysis15.07BBa_K112808Amp
GFP15.07?Amp
dld02.07BBa_K284002Amp
RBS Backbone + VGB03.07 Amp
YFP + DTT11.07 Amp
GFP + DTT11.07 Amp
RFP + DTT11.07 Amp
K+RBS+YFP+DTT17.07 Amp
VGB+RBS+GFP+DTT17.07 Amp
VGB+RBS+RFP+DTT17.07 Amp
pBAD16.07 Amp
Jen1+RBS+GFP+DTT17.07 Amp
LuxR+HSL15.07 Amp
VHB + RBS06.07 Amp
VGB+RBS06.07 Amp
dld+RBS06.07 Amp
RBS backbone + VGB03.07Amp
Jen1+RBS06.07 Amp
LuxR+DTT12.07BBa_C0062+BBa_B0015 Amp
LuxI+DTT12.07 Amp
K+RBS11.07BBa_K081005Amp


Agar plate log: