User talk:Jarlemag




  • Make cloramphenicol stock. Check.
  • Make glycerol stocks of important parts. Discard old plates. Check.
  • Perform CFU-assay of lysis induced cells. Check. Redo?
  • Combine LuxR-HSL and lysis device.
  • Combine pBAD and GFP
  • Research lactate production in E. coli
  • Write more for the book chapter
  • Research QuikChange

Useful links'

Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits

Structural Biology Target Registration Database

DNASU plasmid repository

Addgene plasmid repository

PlasmID database

Moss lab protocols

Lactate genes and promoters:

Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate

Characterization of a Corynebacterium glutamicum Lactate Utilization Operon Induced during Temperature-Triggered Glutamate Production†

KEGG: cg1027

[ The ldhA Gene, Encoding Fermentative l-Lactate Dehydrogenase of Corynebacterium glutamicum, Is under the Control of Positive Feedback Regulation Mediated by LldR

Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif

Promoters of Corynebacterium glutamicum

C. glutamicum lldR crystal structure

RNA polymerase:

Oriented, active Escherichia coli RNA polymerase: an atomic force microscope study

Sequence Specific Interaction between Promoter DNA and Escherichia coli RNA Polymerase: Comparative Thermodynamic Analysis with One Immobilized Partner

Rapid purification of His(6)-tagged Bacillus subtilis core RNA polymerase

Wang et al, 1995: Discontinuous movements of DNA and RNA in RNA polymerase accompany formation of a paused transcription complex

DNA oligo and gene synthesis


PCR Principles and Practices

New England Biolabs: PCR Guidelines

Premier biosoft: PCR primer design guidelines

10 tips for designing PCR primers that work

How to make oligonucleotide primers for PCR


quorum sensing

Quorum sensing in bacteria - Annu. Rev. Microbiol. 2001. 55:165–99

N-acyl-L-homoserine lactone signal interception by Escherichia coli.


Syntetisk biologi: Genmodifisert bakterie drep annan bakterie