Team:HKUST-Hong Kong/Glossary

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Team:HKUST-Hong Kong - 2012.igem.org

Glossary

Signal peptide: transient ‘zip code’ of premature protein which facilitates the secretion of protein in Bacillus subtilis.

YbdN - Signal peptide of protein YbdN in Bacillus subtilis.

ybdN - Gene that codes for YbdN.

YdjM - Signal peptide from protein YdjM in Bacillus subtilis.

ydjM - Gene that codes for YdjM.

BMP2 - Bone morphogenetic protein 2, a.

LytC - B. subtilis inherent cell-wall associated hydrolase mediating cell-wall turnover.

lytC – Gene that codes for the LytC hydrolase.

(EAAAK)n type helical linker – Stiff, long helical linker designed to separate fusion proteins with minimal disturbance to the function of both proteins.

RPMrel: a nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT29 colon carcinoma cells.

FLAG:   8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.

PxylA: The region containing the sigma A binding region for the xylose inducible promoter.

XylR: The transcriptional regulator for PxylA promoter.

pTms: A low efficient constitutive promoter in E. coli and B. subtilis.

EndoA: Endoribonuclease that can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).

ydcE (EndoA): Gene that codes for the EndoA endoribonuclease.

YdcD: Antidote that counteracts the function of EndoA.

ydcD (endB): Gene that codes for the YdcD antidote.