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Team:Goettingen/Focus Groups
From 2012.igem.org
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<h2><b><a name="Focus_Groups#2"></a>#2 - Speed Improvement</b></h2> | <h2><b><a name="Focus_Groups#2"></a>#2 - Speed Improvement</b></h2> | ||
<p align="justify" style="line-height:1.6em"> | <p align="justify" style="line-height:1.6em"> | ||
| - | + | In this group we focus on the improvement of <i>E. coli</i> swimming abilities. So far, we have been working with strain DH10B. | |
</p> | </p> | ||
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<h2><b><a name="Focus_Groups#3"></a>#3 - Chemoreceptor Library</b></h2> | <h2><b><a name="Focus_Groups#3"></a>#3 - Chemoreceptor Library</b></h2> | ||
<p align="justify" style="line-height:1.6em"> | <p align="justify" style="line-height:1.6em"> | ||
| - | + | In this group, the main focus is to first clone the aspartate receptor TAR from <i>E. coli</i> under different strong constitutive promoters | |
| + | into vectors. After examination of effects overexpressing this chemotaxis receptor, the next challenge is to mutagenize this protein to receive | ||
| + | a chemoreceptor library. Ideally, the specificity of the receptor becomes altered due to mutation of the ligand binding sites important for chemical binding. | ||
</p> | </p> | ||
<br> | <br> | ||
Revision as of 21:06, 15 August 2012
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Language:  English,  Deutsch
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Focus GroupsUnder process.. #1 - Selection / SwimmingUnder process... #2 - Speed ImprovementIn this group we focus on the improvement of E. coli swimming abilities. So far, we have been working with strain DH10B. #3 - Chemoreceptor LibraryIn this group, the main focus is to first clone the aspartate receptor TAR from E. coli under different strong constitutive promoters into vectors. After examination of effects overexpressing this chemotaxis receptor, the next challenge is to mutagenize this protein to receive a chemoreceptor library. Ideally, the specificity of the receptor becomes altered due to mutation of the ligand binding sites important for chemical binding. ↑ Return to top
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