Team:TU Darmstadt/Protocols/Affinity chromatography

Affinity chromatography

Procedure

Column preparation

Before use the column needs to be activated:

1. drain the ethanol
2. wash the column with dist. water
3. load the column with 12 mL NiCl₂ solution
4. wash the column with PBS buffer

Now the column is ready to use.

Columns can be reused. In oder to store them:

1. wash the coumn with EDTA buffer
2. discard the flowthrough and wash two times with 20% ethanol
3. close up the column with Parafilm and store at 4°C