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Team:Columbia-Cooper-NYC/Cooper notebook
From 2012.igem.org
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Cooper Union Kanbar Lab Notebook
August, 2012
Week 1
Tuesday, 14th
- Created antibiotic CAM by mixing .250g of 25mg/ml of CAM powder with 10ml of EtOH
- Pipetted 135µl of LB with 15µl of glucose to create growth media and placed in 37°C bath
- Created two plates, one labelled control without IPTG and one with IPTG
- Chemically transformed competent cells (DH5α) with IPTG induced GFP plasmid using bioline protocol
- Plated 10µl of antibiotics, all of the transformed cells and 10µl of IPTG (did not place IPTG for control plate)
- Placed in incubator 37°C overnight
Wednesday, 15th
- Observed no growth for both plates
- Concluded that possible lack of expression could be from a lack of a terminator
