Storing cells in Glycerol Solution
What you need:
- 1.5 mL cryogenic vials
- 50% glycerol solution (can also use 20%)
- An overnight culture of the cells of interest
Procedure:
1. To a cryogenic vial, add 600 uL 50% glycerol solution and 400 uL liquid cell culture
2. Mix thoroughy and store in the -80˚C freezer until use
Unless noted, all procedures are based on those in the lab of Christopher Rao
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