Team:UIUC-Illinois/Notebook/Protocols/Storage

From 2012.igem.org

Revision as of 15:28, 4 June 2012 by Bchen26 (Talk | contribs)

Header

Protocols

Select Protocol

Storing cells in Glycerol Solution


What you need:

- 1.5 mL cryogenic vials
- 50% glycerol solution (can also use 20%)
- An overnight culture of the cells of interest

Procedure:

1. To a cryogenic vial, add 600 uL 50% glycerol solution and 400 uL liquid cell culture
2. Mix thoroughy and store in the -80˚C freezer until use

Unless noted, all procedures are based on those in the lab of Christopher Rao (http://www.scs.illinois.edu/rao/index.php).

Retrieved from "http://2012.igem.org/Team:UIUC-Illinois/Notebook/Protocols/Storage"