Team:UIUC-Illinois/Notebook/Protocols/TAE

Select Protocol

• Bootcamp Protocols
• Subculturing Plates
• Making Electrocompetent E.Coli
• Making TAE Buffers
• Making Electrophoresis Gels
• Gel Purification
• Inoculation
• Miniprep
• PCR Protocols
• Digestions
• Ligations
• Transformation of E.Coli
• Storage of Cells

Buffer Protocols

Making 50X TAE buffer

What you need:

- Tris base
- Glacial acetic acid
- Na2EDTA•2H2O
- MilliQ water

Procedure:

1. In a 1000mL pyrex bottle add and mix: - 242 Tris base
- 57.1mL glacial acetic acid
- 37.2g Na2EDTA•2H2O
- 1000mL MilliQ water
2. Mix thoroughly, no sterilization necessary

Making 1X TAE buffer

What you need:
- 50X TAE buffer
- MilliQ water

Procedure:

1. Add 20mL 50X TAE buffer to 980mL MilliQ water and mix to make 1L of 1X TAE buffer stock