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Team:Evry/Notebook/w6
From 2012.igem.org
Weeks:
| June | July | August | September | October | November | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Week 6: 16th July - 22nd July
Monday, 16th July
Bacterial Transformation:
Transformation of AID + TIR1 containing plasmid into TOP10 bacterias.Thuesday, 17th July
Reception and preparation of pCS2+ primers.Friday, 20th July
PCR Colony:
PCR colony of T10 pSC2+ with RFPPrimers used : SP6 and T3
Site Mutagenesis directed:
PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions SitePrimer used : SdMTIR1-Fw and SdMTIR1-Rv
