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From 2012.igem.org

Weeks:

June			July				August				September				October				November

Week 6: 16th July - 22nd July

Monday, 16th July

Bacterial Transformation:

Transformation of AID + TIR1 containing plasmid into TOP10 bacterias.

Thuesday, 17th July

Reception and preparation of pCS2+ primers.

Friday, 20th July

PCR Colony:

PCR colony of T10 pSC2+ with RFP
Primers used : SP6 and T3

Site Mutagenesis directed:

PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site
Primer used : SdMTIR1-Fw and SdMTIR1-Rv