Team:NTNU Trondheim/Stocks

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Revision as of 18:34, 29 July 2012 by Jarlemag (Talk | contribs)

This page lists stored cultures and other samples:

Dates are in the format dd.mm.

See also Constructs page.

Contents

Stored samples

Resuspended DNA

(Dried DNA from distribution kit resuspended in dH2O)

Name/abbreviation Date stored Description Source Backbone
LR? <partinfo>BBa_C0062</partinfo>(RS)<partinfo>pSB1A2</partinfo>
Lysis22.06 BBa_K112808(RS)<partinfo>pSB1A2</partinfo>
C78?<partinfo>BBa_C0078</partinfo>(RS)<partinfo>pSB2K3</partinfo>
C79?<partinfo>BBa_C0079</partinfo>(RS)<partinfo>pSB2K3</partinfo>
RBS24.06<partinfo>BBa_B0034</partinfo>(RS)<partinfo>pSB1A2</partinfo>
LuxI?<partinfo>BBa_C0061</partinfo>(RS)<partinfo>pSB1A2</partinfo>
K+RBS?<partinfo>BBa_K081005</partinfo>(RS)<partinfo>pSB1A2</partinfo>

Isolated DNA

(Miniprep, gel extraction and PCR products)
NOT included here: Digestion and ligation mixtures/products

Name/abbreviation Date stored Description Concentration Backbone
pTet GFP26.07<partinfo>BBa_I13522</partinfo> <partinfo>pSB1A2</partinfo>
VGB+RBS+YFP+DTT26.07
VHB+RBS+YFP+DTT26.07
EYFP (RBS+ LVA- TERM)26.07<partinfo>BBa_E0430</partinfo> pSB1A2
LuxR+Term16.7<partinfo>BBa_C0062</partinfo> + <partinfo>BBa_B0015</partinfo> 37,0 <partinfo>pSB1AK3</partinfo>
LuxI+Term16.7<partinfo>BBa_C0061</partinfo> + <partinfo>BBa_B0015</partinfo>37,6 <partinfo>pSB1AK3</partinfo>
LI (BBa_K092400)?<partinfo>BBa_K092400</partinfo> <partinfo>pSB1A2</partinfo>
GFP+DTT13.07<partinfo>BBa_K082003</partinfo>+<partinfo>BBa_B0015</partinfo>41,1<partinfo>pSB1AK3</partinfo>
YFP+DTT13.07<partinfo>BBa_E0030</partinfo>+<partinfo>BBa_B0015</partinfo>55,5<partinfo>pSB1AK3</partinfo>
RFP+DTT13.07<partinfo>BBa_E1010</partinfo>+<partinfo>BBa_B0015</partinfo>40,9<partinfo>pSB1AK3</partinfo>
CFP+DTT13.07<partinfo>BBa_E0020</partinfo>+<partinfo>BBa_B0015</partinfo>47,7<partinfo>pSB1AK3</partinfo>
K+RBS13.07<partinfo>BBa_K081005</partinfo>19,8<partinfo>pSB1A2</partinfo>
DLD + RBS09.07<partinfo>BBa_K284003</partinfo> + <partinfo>BBa_B0034</partinfo> 24,1<partinfo>pSB1A2</partinfo>
VHB + RBS09.07<partinfo>BBa_K258005</partinfo> + <partinfo>BBa_B0034</partinfo> 48,3<partinfo>pSB1A2</partinfo>
Jen1 + RBS09.07<partinfo>BBa_K284002</partinfo> + <partinfo>BBa_B0034</partinfo> 35,3<partinfo>pSB1A2</partinfo>
VGB + RBS09.07<partinfo>BBa_K561001</partinfo> + <partinfo>BBa_B0034</partinfo> 40,0<partinfo>pSB1A2</partinfo>
pBAD+lysis09.07 23,6<partinfo>pSB1A3</partinfo>
RBS06.07<partinfo>BBa_B0034</partinfo>44,5<partinfo>pSB1A2</partinfo>
LuxI06.07<partinfo>BBa_C0061</partinfo> 38,6<partinfo>pSB1A2</partinfo>
Colicin04.07<partinfo>BBa_K150009</partinfo>58,6<partinfo>pSB1A2</partinfo>
VHB04.07<partinfo>BBa_K258005</partinfo>44,9<partinfo>pSB1A2</partinfo>
Jen104.07<partinfo>BBa_K284002</partinfo>102,1<partinfo>BBa_J63010</partinfo>
DLD04.07<partinfo>BBa_K284003</partinfo>63,8<partinfo>BBa_J63010</partinfo>
pBAD B101.07<partinfo>BBa_K206000</partinfo>24,0<partinfo>pSB1A3</partinfo>
pBAD B201.07<partinfo>Bba_K206000</partinfo>19<partinfo>pSB1A3</partinfo>
LR?<partinfo>BBa_C0062</partinfo> 20,3<partinfo>pSB1A2</partinfo>
VGB promoter27.06<partinfo>BBa_K561001</partinfo>22,9<partinfo>pSB1C3</partinfo>
C7927.06<partinfo>BBa_C0079</partinfo>70,8<partinfo>pSB2K3</partinfo>
C7827.06<partinfo>BBa_C0078</partinfo>63,4<partinfo>pSB2K3</partinfo>
RBS27.06<partinfo>BBa_B0034</partinfo>30,2<partinfo>pSB1A2</partinfo>
RBS26.06<partinfo>BBa_B0034</partinfo>16,8<partinfo>pSB1A2</partinfo>
DTT Amp26.06<partinfo>BBa_B0015</partinfo>35,8<partinfo>pSB1AK3</partinfo>
DTT Kan26.06<partinfo>BBa_B0015</partinfo>26,5<partinfo>pSB1AK3</partinfo>
VGB DNA25.06<partinfo>BBa_K561001</partinfo> <partinfo>pSB1C3</partinfo>
luxR+HSL25.06<partinfo>BBa_R0062</partinfo> 13,3<partinfo>pSB1A2</partinfo>
Lysis1 DNA24.06<partinfo>BBa_K112808</partinfo> <partinfo>pSB1A2</partinfo>
LuxR+HSL24.06<partinfo>BBa_R0062</partinfo> <partinfo>pSB1A2</partinfo>
DNA lysis24.06<partinfo>BBa_K112808</partinfo> <partinfo>pSB1A2</partinfo>
Constitutive promoter22.06<partinfo>BBa_J23119</partinfo> 27,7<partinfo>pSB1A2</partinfo>
D222.6<partinfo>BBa_K145150</partinfo>23,1<partinfo>pSB1A2</partinfo>
D122.06<partinfo>BBa_K091146</partinfo>19,8<partinfo>pSB1A2</partinfo>
RFP K22.06<partinfo>BBa_E1010</partinfo> <partinfo>pSB2K3</partinfo>
GFP AK22.06<partinfo>BBa_K082003</partinfo> <partinfo>pSB1AK3</partinfo>
CFP A22.06<partinfo>BBa_E0020</partinfo> <partinfo>pSB1A2</partinfo>
YFP AK22.06 <partinfo>BBa_E0030</partinfo> <partinfo>pSB1AK3</partinfo>

Restriction digests and ligation mixtures

(Inserts and backbones, DNA treated with enzymes)


Name/abbreviation Date stored Description Enzymes
YFP+Term (religation)11.07YFP+term (religated)?<partinfo>pSB1AK3</partinfo>
GFP+Term (religation)11.07<partinfo>BBa_K082003</partinfo> + <partinfo>BBa_B0015</partinfo> <partinfo>pSB1AK3</partinfo>
CFP+Term (religation)11.07<partinfo>BBa_E0020</partinfo> + <partinfo>BBa_B0015</partinfo> <partinfo>pSB1AK3</partinfo>
RFP +Term (religation)11.07<partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> <partinfo>pSB1AK3</partinfo>

Glycerol stocks

(Cell cultures kept at -80 C)

Name/abbreviation Date stored Strain Plasmid Position
K+RBS+RFP+DTT?DH5a?C1
K+RBS+GFP+DTT?DH5a?C2
RBS (<partinfo>BBa_B0034</partinfo>)?DH5a?C3
pTet mRFP (<partinfo>BBa_I13521</partinfo>)27.07DH5a<partinfo>pSB1A2</partinfo>C6
pTet GFP (<partinfo>BBa_I13522</partinfo>)27.07DH5a<partinfo>pSB1A2</partinfo>C5
EYFP (RBS+ LVA- TERM) (<partinfo>BBa_E0430</partinfo>)27.07DH5a<partinfo>pSB1A2</partinfo>C4
RFP (<partinfo>BBa_E1010</partinfo>) 18.07 DH5a <partinfo>pSB2K3</partinfo> B6
VGB (<partinfo>BBa_K561001</partinfo>)18.07DH5a<partinfo>pSB1C3</partinfo>B5
LuxR+HSL (<partinfo>BBa_R0062</partinfo>)16.07 DH5a<partinfo>pSB1A2</partinfo>A1
Lysis (<partinfo>BBa_K112808</partinfo>)16.07 DH5a<partinfo>pSB1A2</partinfo>A3
pBAD (<partinfo>Bba_K206000</partinfo>)16.07 DH5a<partinfo>pSB1A3</partinfo>A4
LuxR (<partinfo>BBa_C0062</partinfo>)16.07 DH5a<partinfo>pSB1A2</partinfo>A2
GFP (<partinfo>BBa_K082003</partinfo>)16.07 DH5a<partinfo>pSB1AK3</partinfo>A5
pBAD+Lysis16.07 DH5a<partinfo>pSB1A3</partinfo> A6
DTT (<partinfo>BBa_B0015</partinfo>17.07 DH5a<partinfo>pSB1AK3</partinfo> B1
D2 (<partinfo>BBa_K145150</partinfo>)17.07 DH5a<partinfo>pSB1A2</partinfo> B4
CFP (<partinfo>BBa_E0020</partinfo>)17.07 DH5a<partinfo>pSB1A2</partinfo> B2
YFP (<partinfo>BBa_E0030</partinfo>)17.07 DH5a<partinfo>pSB1AK3</partinfo>B3

PCR Products

Name Target Primers Template Status
pllDpllD promoter K-12Gel OK. Ligated and transformed, but unconfirmed.
ColicinColicin operon ?Gel good - strong band.


Agar plates

Name/abbreviation Date stored Description AntibioticPlasmid
VHB+RBS+YFP+DTT24.07VHB test constructAmp
VGB+RBS+YFP+DTT24.07VGB test constructAmp
RBS backbone03.07 ? Amp
GFP + term (DTT)11.7 Amp
Jen102.07<partinfo>BBa_K284002</partinfo>Amp
BBa_B003422.7RBSAmp
VHB02.07<partinfo>BBa_K258005</partinfo>Amp
LuxR15.07<partinfo>BBa_C0061</partinfo>Amp
pBAD + lysis15.07<partinfo>Bba_K206000</partinfo>+<partinfo>BBa_K112808</partinfo>Amp
Lysis15.07<partinfo>BBa_K112808</partinfo>Amp
GFP15.07?Amp
dld02.07<partinfo>BBa_K284002</partinfo>Amp
RBS Backbone + VGB03.07 Amp
YFP + DTT11.07 Amp
GFP + DTT11.07 Amp
RFP + DTT11.07 Amp
K+RBS+YFP+DTT17.07 Amp
VGB+RBS+GFP+DTT17.07 Amp
VGB+RBS+RFP+DTT17.07 Amp
pBAD16.07 Amp
Jen1+RBS+GFP+DTT17.07 Amp
LuxR+HSL15.07 Amp
Retrieved from "http://2012.igem.org/Team:NTNU_Trondheim/Stocks"