Team:Evry/Notebook/July/23

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Promoters & Reporters workgroup

4 different clones pCS2+ RFP => incubation in LB medium overnight at 37 degree celsius.


PCR of pCS2+:

Réactifs Volumes (µl)
GC Buffer 10
dNTPs 1
DNA 1
H2O 32,5
Primers (FW and RV) 2,5 each
Phusion polymerase 0,5