From 2012.igem.org
Promoters & Reporters workgroup
4 different clones pCS2+ RFP => incubation in LB medium overnight at 37 degree celsius.
PCR of pCS2+:
Réactifs |
Volumes (µl) |
GC Buffer |
10 |
dNTPs |
1 |
DNA |
1 |
H2O |
32,5 |
Primers (FW and RV) |
2,5 each |
Phusion polymerase |
0,5 |