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From 2012.igem.org

Promoters & Reporters workgroup

4 different clones pCS2+ RFP => incubation in LB medium overnight at 37 degree celsius.

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PCR of pCS2+:

Réactifs	Volumes (µl)
GC Buffer	10
dNTPs	1
DNA	1
H2O	32,5
Primers (FW and RV)	2,5 each
Phusion polymerase	0,5