Ethan's Research: Yeast Genome Integration and Bacterial Secretion of Kumamolisin

Yeast System

• Yeast Vectors 1
• Yeast Vectors 2
• A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces ceratisiae

• GAL1 and GAL10 (galactose catabolism) selection
• TRP1 selection

Prokaryotic System

• BBa-J23100 (strong strength)
• BBa-J23108 (medium strength)
• BBa-J23109 (weak strength)

• BBa-B0015



RBS
• Constitutive

Secretion
• HlyA Gene Sequence
• A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium
• Analysis of the haemolysin transport process through the secretion from Escherichia coli of PCM, CAT or beta-galactosidase fused to the Hly C-terminal signal domain
• Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins

Kumamolisin Info
• 57 kDa (beta-galactosidase: 540 kDa; source)
• 1701 bp
• 567 aa