From 2012.igem.org
Reporter + TIR1 groups
'''PCR Colony'''
PCR colony of T10 pSC2+ with RFP
''Primers used'' : SP6 and T3
'''Site Mutagenesis directed'''
PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site
''Primer used'' : SdMTIR1-Fw and SdMTIR1-Rv