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From 2012.igem.org

Reporter + TIR1 groups

'''PCR Colony'''

PCR colony of T10 pSC2+ with RFP
Primers used : SP6 and T3


'''Site Mutagenesis directed'''
PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site
Primer used : SdMTIR1-Fw and SdMTIR1-Rv