Team:METU

2. Human Voice Induced Gene Circuit Using Electrically Induced Promoters:

In this project, our aim is to use human voice as a signal, and stimulate a biological system in bacteria by this signal. For this purpose, first human voice is converted to electrical signals by an designed electronic device. In the second part of the project electrical signals coming from electronical device stimulate a promoter and induce a gene expression. According to frequency of voice, different currents are created by device, and we will try to activate different systems in bacteria by different currents.

To see whether this system will work or not, we are planning to use marker genes (such as GFP, RFP or luciferase) as activated by electric stimulus. If we succeed to run this system, the future perspectives are to put more complicated gene circuits under this mechanism.

We will use Bacillus Subtilis 168 strain for this project since it is more resistant to environmental stress than E.coli.