Team:KAIST Korea/Notebook Labnote/2012 10 temp
From 2012.igem.org
![](https://static.igem.org/mediawiki/2012/5/59/Logopart_Kaist.png)
![](https://static.igem.org/mediawiki/2012/6/61/Home_kaist_background.png)
2012 KAIST Korea
Mail : kaist.igem.2012@gmail.com
Twitter : twitter.com/KAIST_iGEM_2012
Facebook : www.facebook.com/KAISTiGEM2012
Notebook : Labnote-October
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
![](https://static.igem.org/mediawiki/2012/9/95/Starter_gradient_kaist.png)
Labnote
OctoberOctober 1st 2012
No Special Event!
Back to the CalendarOctober 2nd 2012
No Special Event!
Back to the CalendarOctober 3rd 2012
No Special Event!
Back to the CalendarOctober 4th 2012
No Special Event!
Back to the CalendarOctober 5th 2012
No Special Event!
Back to the CalendarOctober 6th 2012
No Special Event!
Back to the CalendarOctober 7th 2012
No Special Event!
Back to the CalendarOctober 8th 2012
No Special Event!
Back to the CalendarOctober 9th 2012
No Special Event!
Back to the CalendarOctober 10th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
LuxI and Xis OE template preparation
Results
![2_1010Fig1](https://static.igem.org/mediawiki/2012/3/39/KAIST_LABNOTE101012_Fig1.png)
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
pAutoIntegrase cloning check and Xis template vector prep
Results
pAutoIntegrase 1-10 – HindIII cut
pGEM-Xis 1-2 – EcoRI cut
![2_1010Fig2](https://static.igem.org/mediawiki/2012/4/4f/KAIST_LABNOTE101012_Fig2.png)
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
insert DNA preparation with OE PCR
Results
┗ pAutoSimple(1-3) and pAutoSimpleInverted(4-6)
┗ pAutoSimple and pAutoSimpleInverted (1 and 2, respectively)/ pAuto(1-3) and pAutoInverted(4-6)
Back to the Calendar![2_1010Fig3](https://static.igem.org/mediawiki/2012/d/df/KAIST_LABNOTE101012_Fig3.png)
![2_1010Fig4](https://static.igem.org/mediawiki/2012/7/7d/KAIST_LABNOTE101012_Fig4.png)
October 11th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
Additional templates
Results
Back to the Calendar![2_1011Fig1](https://static.igem.org/mediawiki/2012/2/2d/KAIST_LABNOTE101112_Fig1.png)
┗ Integrase and Excisionase template
October 12th 2012
No Special Event!
Back to the CalendarOctober 13th 2012
No Special Event!
Back to the CalendarOctober 14th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAutoIntegrase Cloning
Insert DNA – OE PCR
Results
Back to the Calendar![2_1014Fig1](https://static.igem.org/mediawiki/2012/1/12/KAIST_LABNOTE101412_Fig1.png)
Cloning into pSB4A5 is performed with gel extracted DNA fragment.
October 15th 2012
No Special Event!
Back to the CalendarOctober 16th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAutoSimple inserts nested PCR and aiiA template
Results
![2_1016Fig1](https://static.igem.org/mediawiki/2012/c/cb/KAIST_LABNOTE101612_Fig1.png)
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
pAuto, pAutoInverted, pAutoSimple, pAutoSimpleInverted miniprep and RE cut
Results
single digestion with HindIII.
Back to the Calendar![2_1016Fig2](https://static.igem.org/mediawiki/2012/c/c4/KAIST_LABNOTE101612_Fig2.png)
October 17th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAuto OE PCR and Xis, simple bFMO, and bFMO template preparation
Results
sizes ok
![2_1017Fig1](https://static.igem.org/mediawiki/2012/9/9e/KAIST_LABNOTE101712_Fig1.png)
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
pAuto insert DNA preparation
Results
Back to the Calendar![2_1017Fig2](https://static.igem.org/mediawiki/2012/5/56/KAIST_LABNOTE101712_Fig2.png)
intermediate fragment nested PCR
![2_1017Fig3](https://static.igem.org/mediawiki/2012/7/79/KAIST_LABNOTE101712_Fig3.png)
Full pAuto insert DNA construct OE PCR
October 18th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAutoIntegrase enz cut check with NotI
Results
Lane 3, 6, 8 seem to be cloned.
Back to the Calendar![2_1018Fig1](https://static.igem.org/mediawiki/2012/6/66/KAIST_LABNOTE101812_Fig1.png)
October 19th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAutoIntegrase Alternative sequencing with fragments amplification
Results
Back to the Calendar![2_1019Fig1](https://static.igem.org/mediawiki/2012/9/9d/KAIST_LABNOTE101912_Fig1.png)
October 20th 2012
No Special Event!
Back to the CalendarOctober 21st 2012
No Special Event!
Back to the CalendarOctober 22nd 2012
No Special Event!
Back to the CalendarOctober 23rd 2012
No Special Event!
Back to the CalendarOctober 24th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
OE template preparation
Results
Back to the Calendar![2_1024Fig1](https://static.igem.org/mediawiki/2012/9/96/KAIST_LABNOTE102412_Fig1.png)
gp47, aiiA, LuxI, bFMO for simple and pAuto
![2_1024Fig1](https://static.igem.org/mediawiki/2012/5/52/KAIST_LABNOTE102412_Fig2.png)
promoter and Inverted one
October 25th 2012
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
![](https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png)
![](https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png)
pAuto intermediate fragment OE PCR
Results
![1025Fig1](https://static.igem.org/mediawiki/2012/f/f1/KAIST_LABNOTE102512_Fig1.png)
![](https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png)
OE PCR with new primer
Results
Back to the Calendar![2_1025Fig2](https://static.igem.org/mediawiki/2012/a/a9/KAIST_LABNOTE102512_Fig2.png)
pAutoSimple/inverted
![2_1025Fig2](https://static.igem.org/mediawiki/2012/9/9d/KAIST_LABNOTE102512_Fig3.png)
pAuto/inverted
October 26th 2012
No Special Event!
Back to the CalendarOctober 27th 2012
No Special Event!
Back to the CalendarOctober 28th 2012
No Special Event!
Back to the CalendarOctober 29th 2012
No Special Event!
Back to the CalendarOctober 30th 2012
No Special Event!
Back to the CalendarOctober 31st 2012
No Special Event!
Back to the Calendar