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From 2012.igem.org

Project Overview

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Arizona State's 2012 iGEM project aims to develop a portable water-borne pathogen biosensor. The team is looking towards addressing problems that prevent existing biosensor technologies from being effectively used in the field. Specifically, our team is working on a sensor that is cheap, portable, robust easily customizable, and produces a cheap response. Our vision is to build a user-friendly device that does not require any technical expertise to operate.

Figure 1: Biosensor Production Pipeline

Abstract

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Diarrheic pathogens including E.coli O157:H7 serotype, Campylobacter, Shigella, and Salmonella often contaminate drinking water supplies in developing nations and are responsible for approximately 1.5 million worldwide annual deaths. Current technologies for detection of bacteria include DNA hybridization FRET signaling, electrical detection via immobilized antimicrobial peptides, and PCR amplification followed by gel visualization. Our method of bacterial detection fills a niche in biosensor technology. Our design implies lower costs, higher portability, and a more rapid signal output than most bacterial biosensors. Additionally, our interchangeable DNA probe confers modularity, allowing for a range of bacterial detection. Using a novel split beta-galactosidase complementation assay, we have designed three unique chimeric proteins that recognize and bind to specific pathogenic markers and create a functioning beta-galactosidase enzyme. This functioning enzyme unit then cleaves X-gal and produces a colorimetric output signal. Our research demonstrates success in initial stages of chimeric protein assembly.

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