Team:Columbia-Cooper-NYC/Plates

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Protocol for Creating Plates

  • The following is the protocol for creating plates to grow bacteria on:
  1. Prepare 1L bottle
  2. For 500mL, add 7.5g of agar and 12.5g of LB
  3. Pour deionized water until water level reaches 500mL
  4. Cover bottle with foil and tape with autoclave tape
  5. Autoclave on slow for 20 minutes
  6. After autoclaving, let bottle stand cool until touch
  7. Apply 500µl of 1000x stock antibiotics to make 1x solution
  8. Pour solution into plates

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