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Team:Arizona State/Data
From 2012.igem.org
Note from Dr. Haynes: Hi Nisarg
Please add an outline of the experiments-in-progress on the "Data" page. For instance…
Experiment 1
Aim: To determine that the split Beta-gal part of the biosensor works
Approach: Co-express different versions of the alpha and omega fragments in E. coli
Expected Results: If it works as expected, alpha and omega pairs will give a blue phenotype, and single units (negative controls) will not
Lead experimenter: Abhi
Progress: (to be filled in by Abhi)
Experiment 2
Aim: To quantitate Topo enzyme DNA nicking using a crazy sequencing approach
Etc….
Data
ssDNA Probe Design
Topoisomerase-based DNA Biosensor
insert diagram here
Data For Our New Favorite Parts
D168A Double Cysteine Mutant of Smallpox Topoisomerase, BBa_K891234 - This mutant version of topoisomerase recognizes the YCCTT motif in dsDNA. It cleaves after the last T in this motif, making a single stranded nick, and covalently binds to the 3' phosphate on that thymine.
GFPT1, BBa_K891000 - This part should be paired with GFPT2. This part codes for a 20bp sequence that is complementary to a portion of the genomic GFP coding sequence in E.coli Keio strains.
GFPT2, BBa_K891999 - This part should be paired with GFPT1. This part codes for a 20bp sequence that is complementary to a portion of the genomic GFP coding sequence that comes after the GFPT1 binding site in E.coli Keio strains.
