From 2012.igem.org
July 2012
S M T W T F S
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August 2012
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September 2012
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October 2012
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June 07
Transformation (LSE)
Transformed DNA:
lacZ (well 4:12G, I732019)
p + lacO (well 1:6G, R0011)
Cells: neb10beta (donated)
Protocol from: http://www.neb.com/nebecomm/products/productc3019.asp
Controls: puc19, no DNA (8 plates)
June 08
Transformation results
puc19: growth
negative control: no growth
lacZ, lacO: possible small colonies
liquid culture in amp media (100 ug / ml):
no growth of lacZ, lacO
growth of puc19
June 12
DH5a Competent Cell Prep
Streak plated cells on LB no amp plate, let grow overnight
June 13
Transformation (LSE)
Transformed DNA:
lacZ (well 4:12G, I732019)
p + lacO (well 1:6G, R0011)
Cells: DH5 alpha (donated)
Protocol from: http://openwetware.org/wiki/Haynes:Assembly101 (30 minute transformation)
Controls: puc19, no DNA (8 plates)
Transformation (istb4, Abhi)
Transformed DNA:
Cells:
Protocol from:
Controls:
DH5a Chemically Competent cell prep
Grew 2 seed colonies from streak plate in LB no amp
Grew controls to test for contamination
Both Seed colonies grew, no contamination present
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