Team:UANL Mty-Mexico/Notebook

Notebook

Fusion proteins

We used a cloning strategy similar to the Standard Assembly 21 based on the compatible restriction sites BglII and BamHI. A scar is obtained which translated result in the benefical aminoacids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allow us to use the Standard Assembly 10 backbones.

Figure 1. Cloning strategy for contructions of fusion proteins.