Our new biobrick part
FlipFlop(BBa_K907002 and BBa_K907003) is a noble device which can generate binary signal as its name tells us.
This part is composed of three sub-parts.
Signal 0
The FlipFlop, initially, promotes the transcription and translation of down-stream gene, Protein A in the Figure 2, due to its promoter orientation.
When
BBa_K907000(Mycobacteriophage Bxb1 integrase, Bxb1_Int) recognizes and inverts the sequence flanked with attB and attP sequences, promoter orientation is reversed, leaving recombined attL and attR sequences.
Signal 1
Then this device promotes the transcription and translation of upstream gene(must be designed in reverse orientation at construction step), or Protein B(Signal 1) in Figure 4.
Also, the signal can be restored to its initial state when
BBa_K907000/
BBa_K907001 (Mycobacterio-phage Bxb1 excisionase, Bxb1_Xis) complex recognizes and inverts the sequence flanked with attL and attR sequences back to original state.
"
