Team:TU Darmstadt/Protocols

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Protocols

The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012. The corresponding materials can be found in the materials section.

Contents

In vivo

[http://en.wikipedia.org/wiki/E._coli Escherichia coli]

Comamonas testosteroni

  • [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]

In vitro

Nucleic acids

[http://en.wikipedia.org/wiki/DNA DNA]

Protein

In silico

Data analysis

  • Information theory
  • [http://en.wikipedia.org/wiki/R_%28programming_language%29 R Programming]

Databases

  • [http://www.rcsb.org/pdb/home/home.do PDB]
  • [http://www.cas.org/ CAS]

Modelling

Sequence analysis

  • [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST]
  • SAGE

Structure analysis

Material Science

Miscellaneous

Cell culture

Staining

Analytics

ILDs / Improvised Lab Devices